Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration

Abstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while...

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Autores principales:	Anuya Nisal, Raeesa Sayyad, Prachi Dhavale, Bhakti Khude, Rucha Deshpande, Vidhyashri Mapare, Swati Shukla, Premnath Venugopalan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/704930cc48c2457f8c58276fe8eea5c7
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spelling	oai:doaj.org-article:704930cc48c2457f8c58276fe8eea5c72021-12-02T12:32:35ZSilk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration10.1038/s41598-018-25643-x2045-2322https://doaj.org/article/704930cc48c2457f8c58276fe8eea5c72018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25643-xhttps://doaj.org/toc/2045-2322Abstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.Anuya NisalRaeesa SayyadPrachi DhavaleBhakti KhudeRucha DeshpandeVidhyashri MapareSwati ShuklaPremnath VenugopalanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
description	Abstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.
format	article
author	Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan
author_facet	Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan
author_sort	Anuya Nisal
title	Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
title_short	Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
title_full	Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
title_fullStr	Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
title_full_unstemmed	Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
title_sort	silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/704930cc48c2457f8c58276fe8eea5c7
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Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration

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