Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface

Abstract Pollen tubes are used as models in studies on the type of tip-growth in plants. They are an example of polarised and rapid growth because pollen tubes are able to quickly invade the flower pistil in order to accomplish fertilisation. How different ionic fluxes are perceived, processed or ge...

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Autores principales:	Mariusz Pietruszka, Monika Olszewska, Lukasz Machura, Edward Rówiński
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/705a7864a7754a37a150d58db8dc07b0
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spelling	oai:doaj.org-article:705a7864a7754a37a150d58db8dc07b02021-12-02T15:09:05ZSingle measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface10.1038/s41598-018-26015-12045-2322https://doaj.org/article/705a7864a7754a37a150d58db8dc07b02018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26015-1https://doaj.org/toc/2045-2322Abstract Pollen tubes are used as models in studies on the type of tip-growth in plants. They are an example of polarised and rapid growth because pollen tubes are able to quickly invade the flower pistil in order to accomplish fertilisation. How different ionic fluxes are perceived, processed or generated in the pollen tube is still not satisfactorily understood. In order to measure the H+, K+, Ca2+ and Cl− fluxes of a single pollen tube, we developed an Electrical Lab on a Photovoltaic-Chip (ELoPvC) on which the evolving cell was immersed in an electrolyte of a germination medium. Pollen from Hyacinthus orientalis L. was investigated ex vivo. We observed that the growing cell changed the (redox) potential in the medium in a periodic manner. This subtle measurement was feasible due to the effects that were taking place at the semiconductor-liquid interface. The experiment confirmed the existence of the ionic oscillations that accompany the periodic extension of pollen tubes, thereby providing – in a single run – the complete discrete frequency spectrum and phase relationships of the ion gradients and fluxes, while all of the metabolic and enzymatic functions of the cell life cycle were preserved. Furthermore, the global 1/f α characteristic of the power spectral density, which corresponds to the membrane channel noise, was found.Mariusz PietruszkaMonika OlszewskaLukasz MachuraEdward RówińskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariusz Pietruszka Monika Olszewska Lukasz Machura Edward Rówiński Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
description	Abstract Pollen tubes are used as models in studies on the type of tip-growth in plants. They are an example of polarised and rapid growth because pollen tubes are able to quickly invade the flower pistil in order to accomplish fertilisation. How different ionic fluxes are perceived, processed or generated in the pollen tube is still not satisfactorily understood. In order to measure the H+, K+, Ca2+ and Cl− fluxes of a single pollen tube, we developed an Electrical Lab on a Photovoltaic-Chip (ELoPvC) on which the evolving cell was immersed in an electrolyte of a germination medium. Pollen from Hyacinthus orientalis L. was investigated ex vivo. We observed that the growing cell changed the (redox) potential in the medium in a periodic manner. This subtle measurement was feasible due to the effects that were taking place at the semiconductor-liquid interface. The experiment confirmed the existence of the ionic oscillations that accompany the periodic extension of pollen tubes, thereby providing – in a single run – the complete discrete frequency spectrum and phase relationships of the ion gradients and fluxes, while all of the metabolic and enzymatic functions of the cell life cycle were preserved. Furthermore, the global 1/f α characteristic of the power spectral density, which corresponds to the membrane channel noise, was found.
format	article
author	Mariusz Pietruszka Monika Olszewska Lukasz Machura Edward Rówiński
author_facet	Mariusz Pietruszka Monika Olszewska Lukasz Machura Edward Rówiński
author_sort	Mariusz Pietruszka
title	Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
title_short	Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
title_full	Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
title_fullStr	Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
title_full_unstemmed	Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
title_sort	single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/705a7864a7754a37a150d58db8dc07b0
work_keys_str_mv	AT mariuszpietruszka singlemeasurementdetectionofindividualcellionicoscillationsusinganntypesemiconductorelectrolyteinterface AT monikaolszewska singlemeasurementdetectionofindividualcellionicoscillationsusinganntypesemiconductorelectrolyteinterface AT lukaszmachura singlemeasurementdetectionofindividualcellionicoscillationsusinganntypesemiconductorelectrolyteinterface AT edwardrowinski singlemeasurementdetectionofindividualcellionicoscillationsusinganntypesemiconductorelectrolyteinterface
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Single measurement detection of individual cell ionic oscillations using an n-type semiconductor – electrolyte interface

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