Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.

<h4>Rationale</h4>The cardiovascular risk factor homocysteine is mainly bound to proteins in human plasma, and it has been hypothesized that homocysteinylated proteins are important mediators of the toxic effects of hyperhomocysteinemia. It has been recently demonstrated that homocystein...

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Autores principales:	Rosanna Capasso, Irene Sambri, Amelia Cimmino, Sofia Salemme, Cinzia Lombardi, Filomena Acanfora, Ersilia Satta, Donald L Puppione, Alessandra F Perna, Diego Ingrosso
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/705adc8b48ba4991ab3b26a90e756074
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spelling	oai:doaj.org-article:705adc8b48ba4991ab3b26a90e7560742021-11-18T07:28:51ZHomocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.1932-620310.1371/journal.pone.0031388https://doaj.org/article/705adc8b48ba4991ab3b26a90e7560742012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22319627/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Rationale</h4>The cardiovascular risk factor homocysteine is mainly bound to proteins in human plasma, and it has been hypothesized that homocysteinylated proteins are important mediators of the toxic effects of hyperhomocysteinemia. It has been recently demonstrated that homocysteinylated proteins are elevated in hemodialysis patients, a high cardiovascular risk population, and that homocysteinylated albumin shows altered properties.<h4>Objective</h4>Aim of this work was to investigate the effects of homocysteinylated albumin - the circulating form of this amino acid, utilized at the concentration present in uremia - on monocyte adhesion to a human endothelial cell culture monolayer and the relevant molecular changes induced at both cell levels.<h4>Methods and results</h4>Treated endothelial cells showed a significant increase in monocyte adhesion. Endothelial cells showed after treatment a significant, specific and time-dependent increase in ICAM1 and VCAM1. Expression profiling and real time PCR, as well as protein analysis, showed an increase in the expression of genes encoding for chemokines/cytokines regulating the adhesion process and mediators of vascular remodeling (ADAM17, MCP1, and Hsp60). The mature form of ADAM17 was also increased as well as Tnf-α released in the cell medium. At monocyte level, treatment induced up-regulation of ICAM1, MCP1 and its receptor CCR2.<h4>Conclusions</h4>Treatment with homocysteinylated albumin specifically increases monocyte adhesion to endothelial cells through up-regulation of effectors involved in vascular remodeling.Rosanna CapassoIrene SambriAmelia CimminoSofia SalemmeCinzia LombardiFilomena AcanforaErsilia SattaDonald L PuppioneAlessandra F PernaDiego IngrossoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31388 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rosanna Capasso Irene Sambri Amelia Cimmino Sofia Salemme Cinzia Lombardi Filomena Acanfora Ersilia Satta Donald L Puppione Alessandra F Perna Diego Ingrosso Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
description	<h4>Rationale</h4>The cardiovascular risk factor homocysteine is mainly bound to proteins in human plasma, and it has been hypothesized that homocysteinylated proteins are important mediators of the toxic effects of hyperhomocysteinemia. It has been recently demonstrated that homocysteinylated proteins are elevated in hemodialysis patients, a high cardiovascular risk population, and that homocysteinylated albumin shows altered properties.<h4>Objective</h4>Aim of this work was to investigate the effects of homocysteinylated albumin - the circulating form of this amino acid, utilized at the concentration present in uremia - on monocyte adhesion to a human endothelial cell culture monolayer and the relevant molecular changes induced at both cell levels.<h4>Methods and results</h4>Treated endothelial cells showed a significant increase in monocyte adhesion. Endothelial cells showed after treatment a significant, specific and time-dependent increase in ICAM1 and VCAM1. Expression profiling and real time PCR, as well as protein analysis, showed an increase in the expression of genes encoding for chemokines/cytokines regulating the adhesion process and mediators of vascular remodeling (ADAM17, MCP1, and Hsp60). The mature form of ADAM17 was also increased as well as Tnf-α released in the cell medium. At monocyte level, treatment induced up-regulation of ICAM1, MCP1 and its receptor CCR2.<h4>Conclusions</h4>Treatment with homocysteinylated albumin specifically increases monocyte adhesion to endothelial cells through up-regulation of effectors involved in vascular remodeling.
format	article
author	Rosanna Capasso Irene Sambri Amelia Cimmino Sofia Salemme Cinzia Lombardi Filomena Acanfora Ersilia Satta Donald L Puppione Alessandra F Perna Diego Ingrosso
author_facet	Rosanna Capasso Irene Sambri Amelia Cimmino Sofia Salemme Cinzia Lombardi Filomena Acanfora Ersilia Satta Donald L Puppione Alessandra F Perna Diego Ingrosso
author_sort	Rosanna Capasso
title	Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
title_short	Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
title_full	Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
title_fullStr	Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
title_full_unstemmed	Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
title_sort	homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of mcp1, hsp60 and adam17.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/705adc8b48ba4991ab3b26a90e756074
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Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.

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