Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns

Abstract The rickettsial pathogen Ehrlichia chaffeensis causes a tick-borne disease, human monocytic ehrlichiosis. Mutations within certain genomic locations of the pathogen aid in understanding the pathogenesis and in developing attenuated vaccines. Our previous studies demonstrated that mutations...

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Autores principales:	Chandramouli Kondethimmanahalli, Roman Ganta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/705b5e4c003d4bc283fcb43879ec9b5a
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spelling	oai:doaj.org-article:705b5e4c003d4bc283fcb43879ec9b5a2021-12-02T15:08:36ZImpact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns10.1038/s41598-018-24471-32045-2322https://doaj.org/article/705b5e4c003d4bc283fcb43879ec9b5a2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24471-3https://doaj.org/toc/2045-2322Abstract The rickettsial pathogen Ehrlichia chaffeensis causes a tick-borne disease, human monocytic ehrlichiosis. Mutations within certain genomic locations of the pathogen aid in understanding the pathogenesis and in developing attenuated vaccines. Our previous studies demonstrated that mutations in different genomic sites in E. chaffeensis caused variable impacts on their growth and attenuation in vertebrate and tick hosts. Here, we assessed the effect of three mutations on transcriptional changes using RNA deep-sequencing technology. RNA sequencing aided in detecting 66–80% of the transcripts of wildtype and mutant E. chaffeensis. Mutation in an antiporter gene (ECH_0379) causing attenuated growth in vertebrate hosts resulted in the down regulation of many transcribed genes. Similarly, a mutation downstream to the ECH_0490 coding sequence resulted in minimal impact on the pathogen’s in vivo growth, but caused major changes in its transcriptome. This mutation caused enhanced expression of several host stress response genes. Even though the ECH_0660 gene mutation caused the pathogen’s rapid clearance in vertebrate hosts and aids in generating a protective response, there was minimal impact on the transcriptome. The transcriptomic data offer novel insights about the impact of mutations on global gene expression and how they may contribute to the pathogen’s resistance and/or clearance from the host.Chandramouli KondethimmanahalliRoman GantaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chandramouli Kondethimmanahalli Roman Ganta Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
description	Abstract The rickettsial pathogen Ehrlichia chaffeensis causes a tick-borne disease, human monocytic ehrlichiosis. Mutations within certain genomic locations of the pathogen aid in understanding the pathogenesis and in developing attenuated vaccines. Our previous studies demonstrated that mutations in different genomic sites in E. chaffeensis caused variable impacts on their growth and attenuation in vertebrate and tick hosts. Here, we assessed the effect of three mutations on transcriptional changes using RNA deep-sequencing technology. RNA sequencing aided in detecting 66–80% of the transcripts of wildtype and mutant E. chaffeensis. Mutation in an antiporter gene (ECH_0379) causing attenuated growth in vertebrate hosts resulted in the down regulation of many transcribed genes. Similarly, a mutation downstream to the ECH_0490 coding sequence resulted in minimal impact on the pathogen’s in vivo growth, but caused major changes in its transcriptome. This mutation caused enhanced expression of several host stress response genes. Even though the ECH_0660 gene mutation caused the pathogen’s rapid clearance in vertebrate hosts and aids in generating a protective response, there was minimal impact on the transcriptome. The transcriptomic data offer novel insights about the impact of mutations on global gene expression and how they may contribute to the pathogen’s resistance and/or clearance from the host.
format	article
author	Chandramouli Kondethimmanahalli Roman Ganta
author_facet	Chandramouli Kondethimmanahalli Roman Ganta
author_sort	Chandramouli Kondethimmanahalli
title	Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
title_short	Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
title_full	Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
title_fullStr	Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
title_full_unstemmed	Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns
title_sort	impact of three different mutations in ehrlichia chaffeensis in altering the global gene expression patterns
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/705b5e4c003d4bc283fcb43879ec9b5a
work_keys_str_mv	AT chandramoulikondethimmanahalli impactofthreedifferentmutationsinehrlichiachaffeensisinalteringtheglobalgeneexpressionpatterns AT romanganta impactofthreedifferentmutationsinehrlichiachaffeensisinalteringtheglobalgeneexpressionpatterns
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Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns

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