HILIC-MS/MS Analysis of Adenosine in Patient Blood

Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecul...

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Autores principales:	Christina Virgiliou, Nikolaos Fragakis, Melani Sotiriadou, Vassilios Vassilikos, Spiros Gerou, Georgios Theodoridis, Helen Gika
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adenosine HILIC-MS/MS blood bioanalysis syncope Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/705d99d3923b4ab38bd9cffb4bd6429f
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id	oai:doaj.org-article:705d99d3923b4ab38bd9cffb4bd6429f
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spelling	oai:doaj.org-article:705d99d3923b4ab38bd9cffb4bd6429f2021-11-25T18:59:28ZHILIC-MS/MS Analysis of Adenosine in Patient Blood10.3390/separations81102222297-8739https://doaj.org/article/705d99d3923b4ab38bd9cffb4bd6429f2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/222https://doaj.org/toc/2297-8739Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecule’s instability. In the present study, we report a simple method for the pre-treatment of blood samples and the development of a fast and efficient hydrophilic interaction chromatographic tandem mass spectrometry method for the analysis of adenosine in patient blood. During collection, samples were mixed directly with a solvent mixture containing 95% acetonitrile and 10 mM ammonium formate in a Vacutainer tube, resulting in successful prevention of adenosine metabolic processes and direct blood sample deproteinization. The method was validated according to bioanalytical industry guidelines and found to be accurate, repeatable, specific and sensitive with LLOQ 0.005 μg/mL, thus allowing its application in the analysis of real clinical samples.Christina VirgiliouNikolaos FragakisMelani SotiriadouVassilios VassilikosSpiros GerouGeorgios TheodoridisHelen GikaMDPI AGarticleadenosineHILIC-MS/MSbloodbioanalysissyncopePhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 222, p 222 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenosine HILIC-MS/MS blood bioanalysis syncope Physics QC1-999 Chemistry QD1-999
spellingShingle	adenosine HILIC-MS/MS blood bioanalysis syncope Physics QC1-999 Chemistry QD1-999 Christina Virgiliou Nikolaos Fragakis Melani Sotiriadou Vassilios Vassilikos Spiros Gerou Georgios Theodoridis Helen Gika HILIC-MS/MS Analysis of Adenosine in Patient Blood
description	Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecule’s instability. In the present study, we report a simple method for the pre-treatment of blood samples and the development of a fast and efficient hydrophilic interaction chromatographic tandem mass spectrometry method for the analysis of adenosine in patient blood. During collection, samples were mixed directly with a solvent mixture containing 95% acetonitrile and 10 mM ammonium formate in a Vacutainer tube, resulting in successful prevention of adenosine metabolic processes and direct blood sample deproteinization. The method was validated according to bioanalytical industry guidelines and found to be accurate, repeatable, specific and sensitive with LLOQ 0.005 μg/mL, thus allowing its application in the analysis of real clinical samples.
format	article
author	Christina Virgiliou Nikolaos Fragakis Melani Sotiriadou Vassilios Vassilikos Spiros Gerou Georgios Theodoridis Helen Gika
author_facet	Christina Virgiliou Nikolaos Fragakis Melani Sotiriadou Vassilios Vassilikos Spiros Gerou Georgios Theodoridis Helen Gika
author_sort	Christina Virgiliou
title	HILIC-MS/MS Analysis of Adenosine in Patient Blood
title_short	HILIC-MS/MS Analysis of Adenosine in Patient Blood
title_full	HILIC-MS/MS Analysis of Adenosine in Patient Blood
title_fullStr	HILIC-MS/MS Analysis of Adenosine in Patient Blood
title_full_unstemmed	HILIC-MS/MS Analysis of Adenosine in Patient Blood
title_sort	hilic-ms/ms analysis of adenosine in patient blood
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/705d99d3923b4ab38bd9cffb4bd6429f
work_keys_str_mv	AT christinavirgiliou hilicmsmsanalysisofadenosineinpatientblood AT nikolaosfragakis hilicmsmsanalysisofadenosineinpatientblood AT melanisotiriadou hilicmsmsanalysisofadenosineinpatientblood AT vassiliosvassilikos hilicmsmsanalysisofadenosineinpatientblood AT spirosgerou hilicmsmsanalysisofadenosineinpatientblood AT georgiostheodoridis hilicmsmsanalysisofadenosineinpatientblood AT helengika hilicmsmsanalysisofadenosineinpatientblood
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HILIC-MS/MS Analysis of Adenosine in Patient Blood

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