A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress

Antibiotic sensitivity and the effect of temperature on microbial growth are two standard laboratory activities found in most microbial laboratory manuals. We have found a novel way to combine the two activities to demonstrate how temperature can influence antibiotic sensitivity using a standard inc...

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Autores principales:	Donald G. Gerbig, Jean Engohang-Ndong, Heather Aubihl
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/70655498c5f249129e99d8a863336730
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spelling	oai:doaj.org-article:70655498c5f249129e99d8a8633367302021-11-15T15:18:40ZA New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress10.1128/jmbe.v14i2.6171935-78851935-7877https://doaj.org/article/70655498c5f249129e99d8a8633367302013-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v14i2.617https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Antibiotic sensitivity and the effect of temperature on microbial growth are two standard laboratory activities found in most microbial laboratory manuals. We have found a novel way to combine the two activities to demonstrate how temperature can influence antibiotic sensitivity using a standard incubator in instructional laboratory settings. This activity reinforces the important concepts of microbial growth and temperature along with Kirby-Bauer antibiotic susceptibility testing. We found that Pseudomonas fluorescens can be manipulated to become more sensitive to several antibiotics by simply increasing growth temperature and exposing the organism to various antibiotics. No additional equipment is required beyond a standard incubator. Pseudomonas fluorescens is an excellent choice for this activity since it is a safe alternative to Pseudomonas aeruginosa, a biosafety level 2 agent. Pseudomonads are important to explore in the microbiology laboratory since Pseudomonas aeruginosa poses a serious issue in health care settings, as this organism is known to be a multi-drug-resistant pathogen (6). More importantly, P. fluorescens is a good alternative in the laboratory to P. aeruginosa since it is also pigmented (5) and a possible reservoir of antibiotic resistance genes (4). In addition, it grows best at room temperatures and can easily be thermally stressed by placing in a standard 35ºC to 37ºC incubatorDonald G. GerbigJean Engohang-NdongHeather AubihlAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 14, Iss 2, Pp 269-270 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Donald G. Gerbig Jean Engohang-Ndong Heather Aubihl A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
description	Antibiotic sensitivity and the effect of temperature on microbial growth are two standard laboratory activities found in most microbial laboratory manuals. We have found a novel way to combine the two activities to demonstrate how temperature can influence antibiotic sensitivity using a standard incubator in instructional laboratory settings. This activity reinforces the important concepts of microbial growth and temperature along with Kirby-Bauer antibiotic susceptibility testing. We found that Pseudomonas fluorescens can be manipulated to become more sensitive to several antibiotics by simply increasing growth temperature and exposing the organism to various antibiotics. No additional equipment is required beyond a standard incubator. Pseudomonas fluorescens is an excellent choice for this activity since it is a safe alternative to Pseudomonas aeruginosa, a biosafety level 2 agent. Pseudomonads are important to explore in the microbiology laboratory since Pseudomonas aeruginosa poses a serious issue in health care settings, as this organism is known to be a multi-drug-resistant pathogen (6). More importantly, P. fluorescens is a good alternative in the laboratory to P. aeruginosa since it is also pigmented (5) and a possible reservoir of antibiotic resistance genes (4). In addition, it grows best at room temperatures and can easily be thermally stressed by placing in a standard 35ºC to 37ºC incubator
format	article
author	Donald G. Gerbig Jean Engohang-Ndong Heather Aubihl
author_facet	Donald G. Gerbig Jean Engohang-Ndong Heather Aubihl
author_sort	Donald G. Gerbig
title	A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
title_short	A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
title_full	A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
title_fullStr	A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
title_full_unstemmed	A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress
title_sort	new twist to the kirby-bauer antibiotic susceptibility test activity—increasing antibiotic sensitivity of <italic toggle="yes">pseudomonas fluorescens</italic> through thermal stress
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/70655498c5f249129e99d8a863336730
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A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of <italic toggle="yes">Pseudomonas fluorescens</italic> through Thermal Stress

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