The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>

ABSTRACT Ribosomal maturation is a complex and highly conserved biological process involving migration of a continuously changing RNP across multiple cellular compartments. A critical point in this process is the translocation of individual ribosomal subunits (60S and 40S) from the nucleus to the cy...

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Autores principales:	Constance Rink, Martin Ciganda, Noreen Williams
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	nuclear export protein-RNA interactions protein-protein interactions ribosome biogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7069ba11c1584a958b282a54b374dee3
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spelling	oai:doaj.org-article:7069ba11c1584a958b282a54b374dee32021-11-15T15:22:26ZThe Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>10.1128/mSphere.00343-192379-5042https://doaj.org/article/7069ba11c1584a958b282a54b374dee32019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00343-19https://doaj.org/toc/2379-5042ABSTRACT Ribosomal maturation is a complex and highly conserved biological process involving migration of a continuously changing RNP across multiple cellular compartments. A critical point in this process is the translocation of individual ribosomal subunits (60S and 40S) from the nucleus to the cytoplasm, and a number of export factors participate in this process. In this study, we characterize the functional role of the auxiliary export receptors TbMex67 and TbMtr2 in ribosome biogenesis in the parasite Trypanosoma brucei. We demonstrate that depletion of each of these proteins dramatically impacts the steady-state levels of other proteins involved in ribosome biogenesis, including the trypanosome-specific factors P34 and P37. In addition, we observe that the loss of TbMex67 or TbMtr2 leads to aberrant ribosome formation, rRNA processing, and polysome formation. Although the TbMex67-TbMtr2 heterodimer is structurally distinct from Mex67-Mtr2 complexes previously studied, our data show that they retain a conserved function in ribosome biogenesis. IMPORTANCE The nuclear export of ribosomal subunits (60S and 40S) depends in part on the activity of the essential auxiliary export receptors TbMtr2 and TbMex67. When these proteins are individually depleted from the medically and agriculturally significant parasite Trypanosoma brucei, distinct alterations in the processing of the rRNAs of the large subunit (60S) are observed as well as aberrations in the assembly of functional ribosomes (polysomes). We also established that TbMex67 and TbMtr2 interact directly or indirectly with the protein components of the 5S RNP, including the trypanosome-specific P34/P37. The critical role that TbMex67 and TbMtr2 play in this essential biological process together with their parasite-specific interactions may provide new therapeutic targets against this important parasite.Constance RinkMartin CigandaNoreen WilliamsAmerican Society for Microbiologyarticlenuclear exportprotein-RNA interactionsprotein-protein interactionsribosome biogenesisMicrobiologyQR1-502ENmSphere, Vol 4, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	nuclear export protein-RNA interactions protein-protein interactions ribosome biogenesis Microbiology QR1-502
spellingShingle	nuclear export protein-RNA interactions protein-protein interactions ribosome biogenesis Microbiology QR1-502 Constance Rink Martin Ciganda Noreen Williams The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
description	ABSTRACT Ribosomal maturation is a complex and highly conserved biological process involving migration of a continuously changing RNP across multiple cellular compartments. A critical point in this process is the translocation of individual ribosomal subunits (60S and 40S) from the nucleus to the cytoplasm, and a number of export factors participate in this process. In this study, we characterize the functional role of the auxiliary export receptors TbMex67 and TbMtr2 in ribosome biogenesis in the parasite Trypanosoma brucei. We demonstrate that depletion of each of these proteins dramatically impacts the steady-state levels of other proteins involved in ribosome biogenesis, including the trypanosome-specific factors P34 and P37. In addition, we observe that the loss of TbMex67 or TbMtr2 leads to aberrant ribosome formation, rRNA processing, and polysome formation. Although the TbMex67-TbMtr2 heterodimer is structurally distinct from Mex67-Mtr2 complexes previously studied, our data show that they retain a conserved function in ribosome biogenesis. IMPORTANCE The nuclear export of ribosomal subunits (60S and 40S) depends in part on the activity of the essential auxiliary export receptors TbMtr2 and TbMex67. When these proteins are individually depleted from the medically and agriculturally significant parasite Trypanosoma brucei, distinct alterations in the processing of the rRNAs of the large subunit (60S) are observed as well as aberrations in the assembly of functional ribosomes (polysomes). We also established that TbMex67 and TbMtr2 interact directly or indirectly with the protein components of the 5S RNP, including the trypanosome-specific P34/P37. The critical role that TbMex67 and TbMtr2 play in this essential biological process together with their parasite-specific interactions may provide new therapeutic targets against this important parasite.
format	article
author	Constance Rink Martin Ciganda Noreen Williams
author_facet	Constance Rink Martin Ciganda Noreen Williams
author_sort	Constance Rink
title	The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
title_short	The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
title_full	The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
title_fullStr	The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
title_full_unstemmed	The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
title_sort	nuclear export receptors tbmex67 and tbmtr2 are required for ribosome biogenesis in <named-content content-type="genus-species">trypanosoma brucei</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/7069ba11c1584a958b282a54b374dee3
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The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>

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