SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.

SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and...

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Autores principales:	Carlos A Capoano, Luis Adrián Ortiz-Laquintana, Rosana Rodríguez-Casuriaga, Geraldine Schlapp, María Noel Meikle, Ana Paula Mulet, Martina Crispo, Ricardo Benavente, Adriana Geisinger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/706f18dbaffc4fac8bbc7081c49f3039
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spelling	oai:doaj.org-article:706f18dbaffc4fac8bbc7081c49f30392021-11-25T06:19:21ZSPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.1932-620310.1371/journal.pone.0251028https://doaj.org/article/706f18dbaffc4fac8bbc7081c49f30392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251028https://doaj.org/toc/1932-6203SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.Carlos A CapoanoLuis Adrián Ortiz-LaquintanaRosana Rodríguez-CasuriagaGeraldine SchlappMaría Noel MeikleAna Paula MuletMartina CrispoRicardo BenaventeAdriana GeisingerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251028 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos A Capoano Luis Adrián Ortiz-Laquintana Rosana Rodríguez-Casuriaga Geraldine Schlapp María Noel Meikle Ana Paula Mulet Martina Crispo Ricardo Benavente Adriana Geisinger SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
description	SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.
format	article
author	Carlos A Capoano Luis Adrián Ortiz-Laquintana Rosana Rodríguez-Casuriaga Geraldine Schlapp María Noel Meikle Ana Paula Mulet Martina Crispo Ricardo Benavente Adriana Geisinger
author_facet	Carlos A Capoano Luis Adrián Ortiz-Laquintana Rosana Rodríguez-Casuriaga Geraldine Schlapp María Noel Meikle Ana Paula Mulet Martina Crispo Ricardo Benavente Adriana Geisinger
author_sort	Carlos A Capoano
title	SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_short	SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_full	SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_fullStr	SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_full_unstemmed	SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_sort	spats1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/706f18dbaffc4fac8bbc7081c49f3039
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SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.

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