G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells

Abstract Although GPR64 has an important role for male fertility, its physiological roles in the female reproductive system are still unknown. In the present study, immunohistochemical analysis reveals a spatiotemporal expression of GPR64 in the uterus during early pregnancy. Observation of remarkab...

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Autores principales:	Jung-Yoon Yoo, Jong Il Ahn, Tae Hoon Kim, Sungryul Yu, Ji Yeon Ahn, Jeong Mook Lim, Jae-Wook Jeong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/706fcc461a3d44ac90f7a4723082753a
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spelling	oai:doaj.org-article:706fcc461a3d44ac90f7a4723082753a2021-12-02T11:52:37ZG-protein coupled receptor 64 is required for decidualization of endometrial stromal cells10.1038/s41598-017-05165-82045-2322https://doaj.org/article/706fcc461a3d44ac90f7a4723082753a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05165-8https://doaj.org/toc/2045-2322Abstract Although GPR64 has an important role for male fertility, its physiological roles in the female reproductive system are still unknown. In the present study, immunohistochemical analysis reveals a spatiotemporal expression of GPR64 in the uterus during early pregnancy. Observation of remarkable induction of GPR64 expression in uterine decidual cells points to its potential physiological significance on decidualization. The decidualization of uterine stromal cells is a key event in implantation. Progesterone (P4) signaling is crucial for the decidualization of the endometrial stromal cells for successful pregnancy. Therefore, we examined ovarian steroid hormone regulation of GPR64 expression in the murine uterus. P4 induced GPR64 expression in the epithelial and stromal cells of the uterus in ovariectomized wild-type mice, but not in PRKO mice. ChIP analysis confirmed that PGR proteins were recruited on progesterone response element of Gpr64 gene in the uteri of wild-type mice treated with P4. Furthermore, the expression of GPR64 was increased in human endometrial stromal cells (hESCs) during in vitro decidualization. Interestingly, small interfering RNA (siRNA)-mediated knockdown of GPR64 in hESCs remarkably reduced decidualization. These results suggest that Gpr64 has a crucial role in the decidualization of endometrial stromal cells.Jung-Yoon YooJong Il AhnTae Hoon KimSungryul YuJi Yeon AhnJeong Mook LimJae-Wook JeongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jung-Yoon Yoo Jong Il Ahn Tae Hoon Kim Sungryul Yu Ji Yeon Ahn Jeong Mook Lim Jae-Wook Jeong G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
description	Abstract Although GPR64 has an important role for male fertility, its physiological roles in the female reproductive system are still unknown. In the present study, immunohistochemical analysis reveals a spatiotemporal expression of GPR64 in the uterus during early pregnancy. Observation of remarkable induction of GPR64 expression in uterine decidual cells points to its potential physiological significance on decidualization. The decidualization of uterine stromal cells is a key event in implantation. Progesterone (P4) signaling is crucial for the decidualization of the endometrial stromal cells for successful pregnancy. Therefore, we examined ovarian steroid hormone regulation of GPR64 expression in the murine uterus. P4 induced GPR64 expression in the epithelial and stromal cells of the uterus in ovariectomized wild-type mice, but not in PRKO mice. ChIP analysis confirmed that PGR proteins were recruited on progesterone response element of Gpr64 gene in the uteri of wild-type mice treated with P4. Furthermore, the expression of GPR64 was increased in human endometrial stromal cells (hESCs) during in vitro decidualization. Interestingly, small interfering RNA (siRNA)-mediated knockdown of GPR64 in hESCs remarkably reduced decidualization. These results suggest that Gpr64 has a crucial role in the decidualization of endometrial stromal cells.
format	article
author	Jung-Yoon Yoo Jong Il Ahn Tae Hoon Kim Sungryul Yu Ji Yeon Ahn Jeong Mook Lim Jae-Wook Jeong
author_facet	Jung-Yoon Yoo Jong Il Ahn Tae Hoon Kim Sungryul Yu Ji Yeon Ahn Jeong Mook Lim Jae-Wook Jeong
author_sort	Jung-Yoon Yoo
title	G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
title_short	G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
title_full	G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
title_fullStr	G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
title_full_unstemmed	G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
title_sort	g-protein coupled receptor 64 is required for decidualization of endometrial stromal cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/706fcc461a3d44ac90f7a4723082753a
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G-protein coupled receptor 64 is required for decidualization of endometrial stromal cells

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