Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Abstract It is the acquisition of unique traits that adds to the enigma of microbial capabilities to carry out extraordinary processes. One such ecosystem is the soil exposed to radionuclides, in the vicinity of atomic power stations. With the aim to study thorium (Th) tolerance in the indigenous ba...

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Autores principales:	Arpit Shukla, Paritosh Parmar, Dweipayan Goswami, Baldev Patel, Meenu Saraf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7077289dc6ce4e9dadd3dc9629226072
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spelling	oai:doaj.org-article:7077289dc6ce4e9dadd3dc96292260722021-12-02T14:06:31ZExemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM310.1038/s41598-021-82863-42045-2322https://doaj.org/article/7077289dc6ce4e9dadd3dc96292260722021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82863-4https://doaj.org/toc/2045-2322Abstract It is the acquisition of unique traits that adds to the enigma of microbial capabilities to carry out extraordinary processes. One such ecosystem is the soil exposed to radionuclides, in the vicinity of atomic power stations. With the aim to study thorium (Th) tolerance in the indigenous bacteria of such soil, the bacteria were isolated and screened for maximum thorium tolerance. Out of all, only one strain AM3, found to tolerate extraordinary levels of Th (1500 mg L−1), was identified to be belonging to genus Providencia and showed maximum genetic similarity with the type strain P. vermicola OP1T. This is the first report suggesting any bacteria to tolerate such high Th and we propose to term such microbes as ‘thoriotolerant’. The medium composition for cultivating AM3 was optimized using response surface methodology (RSM) which also led to an improvement in its Th-tolerance capabilities by 23%. AM3 was found to be a good producer of EPS and hence one component study was also employed for its optimization. Moreover, the EPS produced by the strain showed interaction with Th, which was deduced by Fourier Transform Infrared (FTIR) spectroscopy.Arpit ShuklaParitosh ParmarDweipayan GoswamiBaldev PatelMeenu SarafNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arpit Shukla Paritosh Parmar Dweipayan Goswami Baldev Patel Meenu Saraf Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
description	Abstract It is the acquisition of unique traits that adds to the enigma of microbial capabilities to carry out extraordinary processes. One such ecosystem is the soil exposed to radionuclides, in the vicinity of atomic power stations. With the aim to study thorium (Th) tolerance in the indigenous bacteria of such soil, the bacteria were isolated and screened for maximum thorium tolerance. Out of all, only one strain AM3, found to tolerate extraordinary levels of Th (1500 mg L−1), was identified to be belonging to genus Providencia and showed maximum genetic similarity with the type strain P. vermicola OP1T. This is the first report suggesting any bacteria to tolerate such high Th and we propose to term such microbes as ‘thoriotolerant’. The medium composition for cultivating AM3 was optimized using response surface methodology (RSM) which also led to an improvement in its Th-tolerance capabilities by 23%. AM3 was found to be a good producer of EPS and hence one component study was also employed for its optimization. Moreover, the EPS produced by the strain showed interaction with Th, which was deduced by Fourier Transform Infrared (FTIR) spectroscopy.
format	article
author	Arpit Shukla Paritosh Parmar Dweipayan Goswami Baldev Patel Meenu Saraf
author_facet	Arpit Shukla Paritosh Parmar Dweipayan Goswami Baldev Patel Meenu Saraf
author_sort	Arpit Shukla
title	Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
title_short	Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
title_full	Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
title_fullStr	Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
title_full_unstemmed	Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3
title_sort	exemplifying an archetypal thorium-eps complexation by novel thoriotolerant providencia thoriotolerans am3
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7077289dc6ce4e9dadd3dc9629226072
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Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

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