A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors

Abstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by a...

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Autores principales:	Leilei Shi, Ankit Rana, Leyla Esfandiari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7079644c2a114d5dacd1585e84e37da4
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spelling	oai:doaj.org-article:7079644c2a114d5dacd1585e84e37da42021-12-02T12:32:10ZA low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors10.1038/s41598-018-25026-22045-2322https://doaj.org/article/7079644c2a114d5dacd1585e84e37da42018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25026-2https://doaj.org/toc/2045-2322Abstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by applying 10 V/cm direct current (DC) across the pipette’s length. The trapping mechanism was systemically studied using both numerical modeling and experimental observations. The results showed that the particle trapping was determined to be controlled by three dominant electrokinetic forces including dielectrophoretic, electrophoretic and electroosmotic force. Furthermore, the effect of the ionic strength, the pipette’s geometry, and the applied electric field on the entrapment efficiency was investigated. To show the application of our device in biomedical sciences, we demonstrated the successful entrapment of fluorescently tagged liposomes and unlabeled plasma-driven exosomes from the PBS solution. Also, to illustrate the selective entrapment capability of our device, 100 nm liposomes were extracted from the PBS solution containing 500 nm polystyrene particles at the tip of the pipette as the voltage polarity was reversed.Leilei ShiAnkit RanaLeyla EsfandiariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leilei Shi Ankit Rana Leyla Esfandiari A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
description	Abstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by applying 10 V/cm direct current (DC) across the pipette’s length. The trapping mechanism was systemically studied using both numerical modeling and experimental observations. The results showed that the particle trapping was determined to be controlled by three dominant electrokinetic forces including dielectrophoretic, electrophoretic and electroosmotic force. Furthermore, the effect of the ionic strength, the pipette’s geometry, and the applied electric field on the entrapment efficiency was investigated. To show the application of our device in biomedical sciences, we demonstrated the successful entrapment of fluorescently tagged liposomes and unlabeled plasma-driven exosomes from the PBS solution. Also, to illustrate the selective entrapment capability of our device, 100 nm liposomes were extracted from the PBS solution containing 500 nm polystyrene particles at the tip of the pipette as the voltage polarity was reversed.
format	article
author	Leilei Shi Ankit Rana Leyla Esfandiari
author_facet	Leilei Shi Ankit Rana Leyla Esfandiari
author_sort	Leilei Shi
title	A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
title_short	A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
title_full	A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
title_fullStr	A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
title_full_unstemmed	A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
title_sort	low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7079644c2a114d5dacd1585e84e37da4
work_keys_str_mv	AT leileishi alowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors AT ankitrana alowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors AT leylaesfandiari alowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors AT leileishi lowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors AT ankitrana lowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors AT leylaesfandiari lowvoltagenanopipettedielectrophoreticdeviceforrapidentrapmentofnanoparticlesandexosomesextractedfromplasmaofhealthydonors
_version_	1718394164388823040

A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors

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