Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells.
Hematopoiesis is a complex process regulated by sets of transcription factors in a stage-specific and context-dependent manner. THAP11 is a transcription factor involved in cell growth, ES cell pluripotency, and embryogenesis. Here we showed that THAP11 was down-regulated during erythroid differenti...
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oai:doaj.org-article:7089654e462a4d59bed8e880ff8f2dd12021-11-18T08:27:50ZEffects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells.1932-620310.1371/journal.pone.0091557https://doaj.org/article/7089654e462a4d59bed8e880ff8f2dd12014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637716/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hematopoiesis is a complex process regulated by sets of transcription factors in a stage-specific and context-dependent manner. THAP11 is a transcription factor involved in cell growth, ES cell pluripotency, and embryogenesis. Here we showed that THAP11 was down-regulated during erythroid differentiation but up-regulated during megakaryocytic differentiation of cord blood CD34+ cells. Overexpression of THAP11 in K562 cells inhibited the erythroid differentiation induced by hemin with decreased numbers of benzidine-positive cells and decreased mRNA levels of α-globin (HBA) and glycophorin A (GPA), and knockdown of THAP11 enhanced the erythroid differentiation. Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. The expression levels of transcription factors such as c-Myc, c-Myb, GATA-2, and Fli1 were changed by THAP11 overexpression. In this way, our results suggested that THAP11 reversibly regulated erythroid and megakaryocytic differentiation.Xiang-Zhen KongRong-Hua YinHong-Mei NingWei-Wei ZhengXiao-Ming DongYang YangFei-Fei XuJian-Jie LiYi-Qun ZhanMiao YuChang-Hui GeJian-Hong ZhangHui ChenChang-Yan LiXiao-Ming YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91557 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Xiang-Zhen Kong Rong-Hua Yin Hong-Mei Ning Wei-Wei Zheng Xiao-Ming Dong Yang Yang Fei-Fei Xu Jian-Jie Li Yi-Qun Zhan Miao Yu Chang-Hui Ge Jian-Hong Zhang Hui Chen Chang-Yan Li Xiao-Ming Yang Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| description |
Hematopoiesis is a complex process regulated by sets of transcription factors in a stage-specific and context-dependent manner. THAP11 is a transcription factor involved in cell growth, ES cell pluripotency, and embryogenesis. Here we showed that THAP11 was down-regulated during erythroid differentiation but up-regulated during megakaryocytic differentiation of cord blood CD34+ cells. Overexpression of THAP11 in K562 cells inhibited the erythroid differentiation induced by hemin with decreased numbers of benzidine-positive cells and decreased mRNA levels of α-globin (HBA) and glycophorin A (GPA), and knockdown of THAP11 enhanced the erythroid differentiation. Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. The expression levels of transcription factors such as c-Myc, c-Myb, GATA-2, and Fli1 were changed by THAP11 overexpression. In this way, our results suggested that THAP11 reversibly regulated erythroid and megakaryocytic differentiation. |
| format |
article |
| author |
Xiang-Zhen Kong Rong-Hua Yin Hong-Mei Ning Wei-Wei Zheng Xiao-Ming Dong Yang Yang Fei-Fei Xu Jian-Jie Li Yi-Qun Zhan Miao Yu Chang-Hui Ge Jian-Hong Zhang Hui Chen Chang-Yan Li Xiao-Ming Yang |
| author_facet |
Xiang-Zhen Kong Rong-Hua Yin Hong-Mei Ning Wei-Wei Zheng Xiao-Ming Dong Yang Yang Fei-Fei Xu Jian-Jie Li Yi-Qun Zhan Miao Yu Chang-Hui Ge Jian-Hong Zhang Hui Chen Chang-Yan Li Xiao-Ming Yang |
| author_sort |
Xiang-Zhen Kong |
| title |
Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| title_short |
Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| title_full |
Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| title_fullStr |
Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| title_full_unstemmed |
Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells. |
| title_sort |
effects of thap11 on erythroid differentiation and megakaryocytic differentiation of k562 cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/7089654e462a4d59bed8e880ff8f2dd1 |
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