Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches

Abstract The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along...

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Autores principales:	Sae-Hae Kim, Byeol-Hee Cho, Hiroshi Kiyono, Yong-Suk Jang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7097a1eb7d0f4cbbba933fffd12284cc
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spelling	oai:doaj.org-article:7097a1eb7d0f4cbbba933fffd12284cc2021-12-02T15:05:58ZMicrobiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches10.1038/s41598-017-02729-62045-2322https://doaj.org/article/7097a1eb7d0f4cbbba933fffd12284cc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02729-6https://doaj.org/toc/2045-2322Abstract The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intestine. Given that PP ILC3s are closely associated with mucosal immune regulation, it is important to characterize the regulatory mechanism of ILC3s. Here, we found that terminal ileal PPs of specific pathogen-free (SPF) mice have fewer NKp46+ ILC3s than jejunal PPs, while there was no difference in NKp46+ ILC3 numbers between terminal ileal and jejunal PPs in antibiotics (ABX)-treated mice. We also found that butyrate levels in the terminal ileal PPs of SPF mice were higher than those in the jejunal PPs of SPF mice and terminal ileal PPs of ABX-treated mice. The reduced number of NKp46+ ILC3s in terminal ileal PPs resulted in a decrease in Csf2 expression and, in turn, resulted in reduced regulatory T cells and enhanced antigen-specific T-cell proliferation. Thus, we suggest that NKp46+ ILC3s are negatively regulated by microbiota-derived butyrate in terminal ileal PPs and the reduced ILC3 frequency is closely associated with antigen-specific immune induction in terminal ileal PPs.Sae-Hae KimByeol-Hee ChoHiroshi KiyonoYong-Suk JangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sae-Hae Kim Byeol-Hee Cho Hiroshi Kiyono Yong-Suk Jang Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
description	Abstract The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intestine. Given that PP ILC3s are closely associated with mucosal immune regulation, it is important to characterize the regulatory mechanism of ILC3s. Here, we found that terminal ileal PPs of specific pathogen-free (SPF) mice have fewer NKp46+ ILC3s than jejunal PPs, while there was no difference in NKp46+ ILC3 numbers between terminal ileal and jejunal PPs in antibiotics (ABX)-treated mice. We also found that butyrate levels in the terminal ileal PPs of SPF mice were higher than those in the jejunal PPs of SPF mice and terminal ileal PPs of ABX-treated mice. The reduced number of NKp46+ ILC3s in terminal ileal PPs resulted in a decrease in Csf2 expression and, in turn, resulted in reduced regulatory T cells and enhanced antigen-specific T-cell proliferation. Thus, we suggest that NKp46+ ILC3s are negatively regulated by microbiota-derived butyrate in terminal ileal PPs and the reduced ILC3 frequency is closely associated with antigen-specific immune induction in terminal ileal PPs.
format	article
author	Sae-Hae Kim Byeol-Hee Cho Hiroshi Kiyono Yong-Suk Jang
author_facet	Sae-Hae Kim Byeol-Hee Cho Hiroshi Kiyono Yong-Suk Jang
author_sort	Sae-Hae Kim
title	Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
title_short	Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
title_full	Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
title_fullStr	Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
title_full_unstemmed	Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
title_sort	microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal peyer’s patches
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7097a1eb7d0f4cbbba933fffd12284cc
work_keys_str_mv	AT saehaekim microbiotaderivedbutyratesuppressesgroup3innatelymphoidcellsinterminalilealpeyerspatches AT byeolheecho microbiotaderivedbutyratesuppressesgroup3innatelymphoidcellsinterminalilealpeyerspatches AT hiroshikiyono microbiotaderivedbutyratesuppressesgroup3innatelymphoidcellsinterminalilealpeyerspatches AT yongsukjang microbiotaderivedbutyratesuppressesgroup3innatelymphoidcellsinterminalilealpeyerspatches
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Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches

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