Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk

Copper (Cu) is an essential element for most living plants, but it is toxic for plants when present in excess. To better understand the response mechanism under excess Cu in plants, especially in flowers, transcriptome sequencing on petunia buds and opened flowers under excess Cu was performed. Inte...

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Autores principales:	Jinglei Wu, Kai Li, Jian Li, Henk Schat, Yanbang Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	petunia petal excess Cu RNA-seq Fe deficiency response photosynthesis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/709979d92ef54d4aa7680e631c45f0cb
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spelling	oai:doaj.org-article:709979d92ef54d4aa7680e631c45f0cb2021-11-11T17:04:53ZTranscriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk10.3390/ijms2221116041422-00671661-6596https://doaj.org/article/709979d92ef54d4aa7680e631c45f0cb2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11604https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Copper (Cu) is an essential element for most living plants, but it is toxic for plants when present in excess. To better understand the response mechanism under excess Cu in plants, especially in flowers, transcriptome sequencing on petunia buds and opened flowers under excess Cu was performed. Interestingly, the transcript level of FIT-independent Fe deficiency response genes was significantly affected in Cu stressed petals, probably regulated by basic-helix-loop-helix 121 (bHLH121), while no difference was found in Fe content. Notably, the expression level of bHLH121 was significantly down-regulated in petals under excess Cu. In addition, the expression level of genes related to photosystem II (PSII), photosystem I (PSI), cytochrome <i>b</i><sub>6</sub>/<i>f</i> complex, the light-harvesting chlorophyll II complex and electron carriers showed disordered expression profiles in petals under excess Cu, thus photosynthesis parameters, including the maximum PSII efficiency (F<sub>V</sub>/F<sub>M</sub>), nonphotochemical quenching (NPQ), quantum yield of the PSII (ΦPS(II)) and photochemical quenching coefficient (qP), were reduced in Cu stressed petals. Moreover, the chlorophyll a content was significantly reduced, while the chlorophyll b content was not affected, probably caused by the increased expression of <i>chlorophyllide a oxygenase</i> (<i>CAO</i>). Together, we provide new insight into excess Cu response and the Cu–Fe crosstalk in flowers.Jinglei WuKai LiJian LiHenk SchatYanbang LiMDPI AGarticlepetuniapetalexcess CuRNA-seqFe deficiency responsephotosynthesisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11604, p 11604 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	petunia petal excess Cu RNA-seq Fe deficiency response photosynthesis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	petunia petal excess Cu RNA-seq Fe deficiency response photosynthesis Biology (General) QH301-705.5 Chemistry QD1-999 Jinglei Wu Kai Li Jian Li Henk Schat Yanbang Li Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
description	Copper (Cu) is an essential element for most living plants, but it is toxic for plants when present in excess. To better understand the response mechanism under excess Cu in plants, especially in flowers, transcriptome sequencing on petunia buds and opened flowers under excess Cu was performed. Interestingly, the transcript level of FIT-independent Fe deficiency response genes was significantly affected in Cu stressed petals, probably regulated by basic-helix-loop-helix 121 (bHLH121), while no difference was found in Fe content. Notably, the expression level of bHLH121 was significantly down-regulated in petals under excess Cu. In addition, the expression level of genes related to photosystem II (PSII), photosystem I (PSI), cytochrome <i>b</i><sub>6</sub>/<i>f</i> complex, the light-harvesting chlorophyll II complex and electron carriers showed disordered expression profiles in petals under excess Cu, thus photosynthesis parameters, including the maximum PSII efficiency (F<sub>V</sub>/F<sub>M</sub>), nonphotochemical quenching (NPQ), quantum yield of the PSII (ΦPS(II)) and photochemical quenching coefficient (qP), were reduced in Cu stressed petals. Moreover, the chlorophyll a content was significantly reduced, while the chlorophyll b content was not affected, probably caused by the increased expression of <i>chlorophyllide a oxygenase</i> (<i>CAO</i>). Together, we provide new insight into excess Cu response and the Cu–Fe crosstalk in flowers.
format	article
author	Jinglei Wu Kai Li Jian Li Henk Schat Yanbang Li
author_facet	Jinglei Wu Kai Li Jian Li Henk Schat Yanbang Li
author_sort	Jinglei Wu
title	Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
title_short	Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
title_full	Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
title_fullStr	Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
title_full_unstemmed	Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk
title_sort	transcriptome profiling of cu stressed petunia petals reveals candidate genes involved in fe and cu crosstalk
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/709979d92ef54d4aa7680e631c45f0cb
work_keys_str_mv	AT jingleiwu transcriptomeprofilingofcustressedpetuniapetalsrevealscandidategenesinvolvedinfeandcucrosstalk AT kaili transcriptomeprofilingofcustressedpetuniapetalsrevealscandidategenesinvolvedinfeandcucrosstalk AT jianli transcriptomeprofilingofcustressedpetuniapetalsrevealscandidategenesinvolvedinfeandcucrosstalk AT henkschat transcriptomeprofilingofcustressedpetuniapetalsrevealscandidategenesinvolvedinfeandcucrosstalk AT yanbangli transcriptomeprofilingofcustressedpetuniapetalsrevealscandidategenesinvolvedinfeandcucrosstalk
_version_	1718432182815424512

Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk

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