Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues

The production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the...

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Autores principales:	Lorenzo Cagnin, Nicoletta Gronchi, Marina Basaglia, Lorenzo Favaro, Sergio Casella
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioethanol sugarcane bagasse cardoon common reed industrial yeast strains steam explosion Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7099e36b9683457a954ce1c4eabf9e61
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spelling	oai:doaj.org-article:7099e36b9683457a954ce1c4eabf9e612021-11-04T09:23:52ZSelection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues1664-302X10.3389/fmicb.2021.756032https://doaj.org/article/7099e36b9683457a954ce1c4eabf9e612021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.756032/fullhttps://doaj.org/toc/1664-302XThe production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the pre-treatment used. It follows that the versatility of the yeast should be considered when selecting a robust strain. This work aimed at the validation of seven natural Saccharomyces cerevisiae strains, previously selected for their industrial fitness, for their application in the production of lignocellulosic bioethanol. Their inhibitor resistance and fermentative performances were compared to those of the benchmark industrial yeast S. cerevisiae Ethanol Red, currently utilized in the second-generation ethanol plants. The yeast strains were characterized for their tolerance using a synthetic inhibitor mixture formulated with increasing concentrations of weak acids and furans, as well as steam-exploded lignocellulosic pre-hydrolysates, generally containing the same inhibitors. The eight non-diluted liquors have been adopted to assess yeast ability to withstand bioethanol industrial conditions. The most tolerant S. cerevisiae Fm17 strain, together with the reference Ethanol Red, was evaluated for fermentative performances in two pre-hydrolysates obtained from cardoon and common reed, chosen for their large inhibitor concentrations. S. cerevisiae Fm17 outperformed the industrial strain Ethanol Red, producing up to 18 and 39 g/L ethanol from cardoon and common reed, respectively, with ethanol yields always higher than those of the benchmark strain. This natural strain exhibits great potential to be used as superior yeast in the lignocellulosic ethanol plants.Lorenzo CagninNicoletta GronchiMarina BasagliaLorenzo FavaroSergio CasellaFrontiers Media S.A.articlebioethanolsugarcane bagassecardooncommon reedindustrial yeast strainssteam explosionMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioethanol sugarcane bagasse cardoon common reed industrial yeast strains steam explosion Microbiology QR1-502
spellingShingle	bioethanol sugarcane bagasse cardoon common reed industrial yeast strains steam explosion Microbiology QR1-502 Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
description	The production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the pre-treatment used. It follows that the versatility of the yeast should be considered when selecting a robust strain. This work aimed at the validation of seven natural Saccharomyces cerevisiae strains, previously selected for their industrial fitness, for their application in the production of lignocellulosic bioethanol. Their inhibitor resistance and fermentative performances were compared to those of the benchmark industrial yeast S. cerevisiae Ethanol Red, currently utilized in the second-generation ethanol plants. The yeast strains were characterized for their tolerance using a synthetic inhibitor mixture formulated with increasing concentrations of weak acids and furans, as well as steam-exploded lignocellulosic pre-hydrolysates, generally containing the same inhibitors. The eight non-diluted liquors have been adopted to assess yeast ability to withstand bioethanol industrial conditions. The most tolerant S. cerevisiae Fm17 strain, together with the reference Ethanol Red, was evaluated for fermentative performances in two pre-hydrolysates obtained from cardoon and common reed, chosen for their large inhibitor concentrations. S. cerevisiae Fm17 outperformed the industrial strain Ethanol Red, producing up to 18 and 39 g/L ethanol from cardoon and common reed, respectively, with ethanol yields always higher than those of the benchmark strain. This natural strain exhibits great potential to be used as superior yeast in the lignocellulosic ethanol plants.
format	article
author	Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella
author_facet	Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella
author_sort	Lorenzo Cagnin
title	Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
title_short	Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
title_full	Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
title_fullStr	Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
title_full_unstemmed	Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
title_sort	selection of superior yeast strains for the fermentation of lignocellulosic steam-exploded residues
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7099e36b9683457a954ce1c4eabf9e61
work_keys_str_mv	AT lorenzocagnin selectionofsuperioryeaststrainsforthefermentationoflignocellulosicsteamexplodedresidues AT nicolettagronchi selectionofsuperioryeaststrainsforthefermentationoflignocellulosicsteamexplodedresidues AT marinabasaglia selectionofsuperioryeaststrainsforthefermentationoflignocellulosicsteamexplodedresidues AT lorenzofavaro selectionofsuperioryeaststrainsforthefermentationoflignocellulosicsteamexplodedresidues AT sergiocasella selectionofsuperioryeaststrainsforthefermentationoflignocellulosicsteamexplodedresidues
_version_	1718444967799554048

Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues

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