Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation

Abstract The degeneration or loss of skeletal muscles, which can be caused by traumatic injury or disease, impacts most aspects of human activity. Among various techniques reported to regenerate skeletal muscle tissue, controlling the external cellular environment has been proven effective in guidin...

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Autores principales:	Hye Kyu Choi, Cheol-Hwi Kim, Sang Nam Lee, Tae-Hyung Kim, Byung-Keun Oh
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Nano-sized graphene oxide Myogenesis Micro−nano hybrid pattern Cell behavior Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999
Acceso en línea:	https://doaj.org/article/709d47edd19d4bc4a3ddd2383e4d3d5a
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spelling	oai:doaj.org-article:709d47edd19d4bc4a3ddd2383e4d3d5a2021-12-05T12:23:48ZNano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation10.1186/s40580-021-00291-62196-5404https://doaj.org/article/709d47edd19d4bc4a3ddd2383e4d3d5a2021-12-01T00:00:00Zhttps://doi.org/10.1186/s40580-021-00291-6https://doaj.org/toc/2196-5404Abstract The degeneration or loss of skeletal muscles, which can be caused by traumatic injury or disease, impacts most aspects of human activity. Among various techniques reported to regenerate skeletal muscle tissue, controlling the external cellular environment has been proven effective in guiding muscle differentiation. In this study, we report a nano-sized graphene oxide (sGO)-modified nanopillars on microgroove hybrid polymer array (NMPA) that effectively controls skeletal muscle cell differentiation. sGO-coated NMPA (sG-NMPA) were first fabricated by sequential laser interference lithography and microcontact printing methods. To compensate for the low adhesion property of polydimethylsiloxane (PDMS) used in this study, graphene oxide (GO), a proven cytophilic nanomaterial, was further modified. Among various sizes of GO, sGO (< 10 nm) was found to be the most effective not only for coating the surface of the NM structure but also for enhancing the cell adhesion and spreading on the fabricated substrates. Remarkably, owing to the micro-sized line patterns that guide cellular morphology to an elongated shape and because of the presence of sGO-modified nanostructures, mouse myoblast cells (C2C12) were efficiently differentiated into skeletal muscle cells on the hybrid patterns, based on the myosin heavy chain expression levels. Therefore, the developed sGO coated polymeric hybrid pattern arrays can serve as a potential platform for rapid and highly efficient in vitro muscle cell generation.Hye Kyu ChoiCheol-Hwi KimSang Nam LeeTae-Hyung KimByung-Keun OhSpringerOpenarticleNano-sized graphene oxideMyogenesisMicro−nano hybrid patternCell behaviorTechnologyTChemical technologyTP1-1185BiotechnologyTP248.13-248.65ScienceQPhysicsQC1-999ENNano Convergence, Vol 8, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nano-sized graphene oxide Myogenesis Micro−nano hybrid pattern Cell behavior Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999
spellingShingle	Nano-sized graphene oxide Myogenesis Micro−nano hybrid pattern Cell behavior Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999 Hye Kyu Choi Cheol-Hwi Kim Sang Nam Lee Tae-Hyung Kim Byung-Keun Oh Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
description	Abstract The degeneration or loss of skeletal muscles, which can be caused by traumatic injury or disease, impacts most aspects of human activity. Among various techniques reported to regenerate skeletal muscle tissue, controlling the external cellular environment has been proven effective in guiding muscle differentiation. In this study, we report a nano-sized graphene oxide (sGO)-modified nanopillars on microgroove hybrid polymer array (NMPA) that effectively controls skeletal muscle cell differentiation. sGO-coated NMPA (sG-NMPA) were first fabricated by sequential laser interference lithography and microcontact printing methods. To compensate for the low adhesion property of polydimethylsiloxane (PDMS) used in this study, graphene oxide (GO), a proven cytophilic nanomaterial, was further modified. Among various sizes of GO, sGO (< 10 nm) was found to be the most effective not only for coating the surface of the NM structure but also for enhancing the cell adhesion and spreading on the fabricated substrates. Remarkably, owing to the micro-sized line patterns that guide cellular morphology to an elongated shape and because of the presence of sGO-modified nanostructures, mouse myoblast cells (C2C12) were efficiently differentiated into skeletal muscle cells on the hybrid patterns, based on the myosin heavy chain expression levels. Therefore, the developed sGO coated polymeric hybrid pattern arrays can serve as a potential platform for rapid and highly efficient in vitro muscle cell generation.
format	article
author	Hye Kyu Choi Cheol-Hwi Kim Sang Nam Lee Tae-Hyung Kim Byung-Keun Oh
author_facet	Hye Kyu Choi Cheol-Hwi Kim Sang Nam Lee Tae-Hyung Kim Byung-Keun Oh
author_sort	Hye Kyu Choi
title	Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
title_short	Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
title_full	Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
title_fullStr	Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
title_full_unstemmed	Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
title_sort	nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/709d47edd19d4bc4a3ddd2383e4d3d5a
work_keys_str_mv	AT hyekyuchoi nanosizedgrapheneoxidecoatednanopillarsonmicrogroovepolymerarraysthatenhanceskeletalmusclecelldifferentiation AT cheolhwikim nanosizedgrapheneoxidecoatednanopillarsonmicrogroovepolymerarraysthatenhanceskeletalmusclecelldifferentiation AT sangnamlee nanosizedgrapheneoxidecoatednanopillarsonmicrogroovepolymerarraysthatenhanceskeletalmusclecelldifferentiation AT taehyungkim nanosizedgrapheneoxidecoatednanopillarsonmicrogroovepolymerarraysthatenhanceskeletalmusclecelldifferentiation AT byungkeunoh nanosizedgrapheneoxidecoatednanopillarsonmicrogroovepolymerarraysthatenhanceskeletalmusclecelldifferentiation
_version_	1718371963041218560

Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation

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