Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells

Chao Wu,1 Yu Gao,2 Ying Liu,1 XiaoYan Xu1 1Pharmacy School, Jinzhou Medical University, Jinzhou, People’s Republic of China; 2Department of Medical Oncology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of China Introduction: Pure paclitax...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wu C, Gao Y, Liu Y, Xu XY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	electrostatic spraying paclitaxel SMMC-7721 cells poorly water-soluble drug dissolution Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/70a06e0e76994bdd8c992cb31afc5f7f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70a06e0e76994bdd8c992cb31afc5f7f
record_format	dspace
spelling	oai:doaj.org-article:70a06e0e76994bdd8c992cb31afc5f7f2021-12-02T08:03:12ZPure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells1178-2013https://doaj.org/article/70a06e0e76994bdd8c992cb31afc5f7f2018-10-01T00:00:00Zhttps://www.dovepress.com/pure-paclitaxel-nanoparticles-preparation-characterization-and-antitum-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chao Wu,1 Yu Gao,2 Ying Liu,1 XiaoYan Xu1 1Pharmacy School, Jinzhou Medical University, Jinzhou, People’s Republic of China; 2Department of Medical Oncology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of China Introduction: Pure paclitaxel nanoparticles (PPN), consisting entirely of drug molecules, were prepared by the electrostatic spraying method as promising candidates for antitumor application. Compared with the traditional preparation method, the advantage of the electrostatic spraying method included high production rates, relatively small particle sizes, and ease of preparation.Materials and methods: Paclitaxel was used to prepared PPN by electrostatic spray. The electrostatic spray device included a constant speed pump with a syringe, a high-voltage power supply, and a metal foil receiver was used to prepare and evaluate PPN. The syringe drew off a certain amount of paclitaxel chloroform solution (150 µg/mL) and was placed on the constant speed injection pump. The dissolution behavior of PPN was evaluated by dissolution test and the presence of paclitaxel in PPN was detected by X-Ray powder diffraction and differential scanning calorimetry. Effect of PPN on SMMC-7721 cells were studied by cell uptake, cell apoptosis and antitumor study.Results: The results of X-ray powder diffraction and differential scanning calorimetry characterization showed that the PPN were in an amorphous state. A dissolution study indicated that PPN have a significantly enhanced dissolution rate of paclitaxel. Moreover, SMMC-7721 tumor cells treated with PPN exhibited a distinctly high uptake rate that promoted cell apoptosis. An in vivo antitumor study demonstrated that PPN had significant antitumor efficacy. Conclusion: All conclusions verified that electrostatic spraying is a potential technology for developing PPN, and PPN can be regarded as a promising treatment for cancer. Keywords: electrostatic spraying, paclitaxel, SMMC-7721 cells, poorly water soluble drug, dissolutionWu CGao YLiu YXu XYDove Medical Pressarticleelectrostatic sprayingpaclitaxelSMMC-7721 cellspoorly water-soluble drugdissolutionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6189-6198 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrostatic spraying paclitaxel SMMC-7721 cells poorly water-soluble drug dissolution Medicine (General) R5-920
spellingShingle	electrostatic spraying paclitaxel SMMC-7721 cells poorly water-soluble drug dissolution Medicine (General) R5-920 Wu C Gao Y Liu Y Xu XY Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
description	Chao Wu,1 Yu Gao,2 Ying Liu,1 XiaoYan Xu1 1Pharmacy School, Jinzhou Medical University, Jinzhou, People’s Republic of China; 2Department of Medical Oncology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of China Introduction: Pure paclitaxel nanoparticles (PPN), consisting entirely of drug molecules, were prepared by the electrostatic spraying method as promising candidates for antitumor application. Compared with the traditional preparation method, the advantage of the electrostatic spraying method included high production rates, relatively small particle sizes, and ease of preparation.Materials and methods: Paclitaxel was used to prepared PPN by electrostatic spray. The electrostatic spray device included a constant speed pump with a syringe, a high-voltage power supply, and a metal foil receiver was used to prepare and evaluate PPN. The syringe drew off a certain amount of paclitaxel chloroform solution (150 µg/mL) and was placed on the constant speed injection pump. The dissolution behavior of PPN was evaluated by dissolution test and the presence of paclitaxel in PPN was detected by X-Ray powder diffraction and differential scanning calorimetry. Effect of PPN on SMMC-7721 cells were studied by cell uptake, cell apoptosis and antitumor study.Results: The results of X-ray powder diffraction and differential scanning calorimetry characterization showed that the PPN were in an amorphous state. A dissolution study indicated that PPN have a significantly enhanced dissolution rate of paclitaxel. Moreover, SMMC-7721 tumor cells treated with PPN exhibited a distinctly high uptake rate that promoted cell apoptosis. An in vivo antitumor study demonstrated that PPN had significant antitumor efficacy. Conclusion: All conclusions verified that electrostatic spraying is a potential technology for developing PPN, and PPN can be regarded as a promising treatment for cancer. Keywords: electrostatic spraying, paclitaxel, SMMC-7721 cells, poorly water soluble drug, dissolution
format	article
author	Wu C Gao Y Liu Y Xu XY
author_facet	Wu C Gao Y Liu Y Xu XY
author_sort	Wu C
title	Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
title_short	Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
title_full	Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
title_fullStr	Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
title_full_unstemmed	Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells
title_sort	pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer smmc-7721 cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/70a06e0e76994bdd8c992cb31afc5f7f
work_keys_str_mv	AT wuc purepaclitaxelnanoparticlespreparationcharacterizationandantitumoreffectforhumanlivercancersmmc7721cells AT gaoy purepaclitaxelnanoparticlespreparationcharacterizationandantitumoreffectforhumanlivercancersmmc7721cells AT liuy purepaclitaxelnanoparticlespreparationcharacterizationandantitumoreffectforhumanlivercancersmmc7721cells AT xuxy purepaclitaxelnanoparticlespreparationcharacterizationandantitumoreffectforhumanlivercancersmmc7721cells
_version_	1718398667429249024

Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells

Ejemplares similares