Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment

Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposur...

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Autores principales: Risa Okada, Shin-ichiro Fujita, Riku Suzuki, Takuto Hayashi, Hirona Tsubouchi, Chihiro Kato, Shunya Sadaki, Maho Kanai, Sayaka Fuseya, Yuri Inoue, Hyojung Jeon, Michito Hamada, Akihiro Kuno, Akiko Ishii, Akira Tamaoka, Jun Tanihata, Naoki Ito, Dai Shiba, Masaki Shirakawa, Masafumi Muratani, Takashi Kudo, Satoru Takahashi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/70a0d1618d87416e809859faa1469440
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Sumario:Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposure prevents muscle atrophy at the molecular level. Our main findings indicated that AG onboard environment prevented changes under microgravity in soleus muscle not only in muscle mass and fiber type composition but also in the alteration of gene expression profiles. In particular, transcriptome analysis suggested that AG condition could prevent the alterations of some atrophy-related genes. We further screened novel candidate genes to reveal the muscle atrophy mechanism from these gene expression profiles. We suggest the potential role of Cacng1 in the atrophy of myotubes using in vitro and in vivo gene transductions. This critical project may accelerate the elucidation of muscle atrophy mechanisms.