Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment

Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposur...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Risa Okada, Shin-ichiro Fujita, Riku Suzuki, Takuto Hayashi, Hirona Tsubouchi, Chihiro Kato, Shunya Sadaki, Maho Kanai, Sayaka Fuseya, Yuri Inoue, Hyojung Jeon, Michito Hamada, Akihiro Kuno, Akiko Ishii, Akira Tamaoka, Jun Tanihata, Naoki Ito, Dai Shiba, Masaki Shirakawa, Masafumi Muratani, Takashi Kudo, Satoru Takahashi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/70a0d1618d87416e809859faa1469440
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:70a0d1618d87416e809859faa1469440
record_format dspace
spelling oai:doaj.org-article:70a0d1618d87416e809859faa14694402021-12-02T13:40:51ZTranscriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment10.1038/s41598-021-88392-42045-2322https://doaj.org/article/70a0d1618d87416e809859faa14694402021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88392-4https://doaj.org/toc/2045-2322Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposure prevents muscle atrophy at the molecular level. Our main findings indicated that AG onboard environment prevented changes under microgravity in soleus muscle not only in muscle mass and fiber type composition but also in the alteration of gene expression profiles. In particular, transcriptome analysis suggested that AG condition could prevent the alterations of some atrophy-related genes. We further screened novel candidate genes to reveal the muscle atrophy mechanism from these gene expression profiles. We suggest the potential role of Cacng1 in the atrophy of myotubes using in vitro and in vivo gene transductions. This critical project may accelerate the elucidation of muscle atrophy mechanisms.Risa OkadaShin-ichiro FujitaRiku SuzukiTakuto HayashiHirona TsubouchiChihiro KatoShunya SadakiMaho KanaiSayaka FuseyaYuri InoueHyojung JeonMichito HamadaAkihiro KunoAkiko IshiiAkira TamaokaJun TanihataNaoki ItoDai ShibaMasaki ShirakawaMasafumi MurataniTakashi KudoSatoru TakahashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Risa Okada
Shin-ichiro Fujita
Riku Suzuki
Takuto Hayashi
Hirona Tsubouchi
Chihiro Kato
Shunya Sadaki
Maho Kanai
Sayaka Fuseya
Yuri Inoue
Hyojung Jeon
Michito Hamada
Akihiro Kuno
Akiko Ishii
Akira Tamaoka
Jun Tanihata
Naoki Ito
Dai Shiba
Masaki Shirakawa
Masafumi Muratani
Takashi Kudo
Satoru Takahashi
Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
description Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposure prevents muscle atrophy at the molecular level. Our main findings indicated that AG onboard environment prevented changes under microgravity in soleus muscle not only in muscle mass and fiber type composition but also in the alteration of gene expression profiles. In particular, transcriptome analysis suggested that AG condition could prevent the alterations of some atrophy-related genes. We further screened novel candidate genes to reveal the muscle atrophy mechanism from these gene expression profiles. We suggest the potential role of Cacng1 in the atrophy of myotubes using in vitro and in vivo gene transductions. This critical project may accelerate the elucidation of muscle atrophy mechanisms.
format article
author Risa Okada
Shin-ichiro Fujita
Riku Suzuki
Takuto Hayashi
Hirona Tsubouchi
Chihiro Kato
Shunya Sadaki
Maho Kanai
Sayaka Fuseya
Yuri Inoue
Hyojung Jeon
Michito Hamada
Akihiro Kuno
Akiko Ishii
Akira Tamaoka
Jun Tanihata
Naoki Ito
Dai Shiba
Masaki Shirakawa
Masafumi Muratani
Takashi Kudo
Satoru Takahashi
author_facet Risa Okada
Shin-ichiro Fujita
Riku Suzuki
Takuto Hayashi
Hirona Tsubouchi
Chihiro Kato
Shunya Sadaki
Maho Kanai
Sayaka Fuseya
Yuri Inoue
Hyojung Jeon
Michito Hamada
Akihiro Kuno
Akiko Ishii
Akira Tamaoka
Jun Tanihata
Naoki Ito
Dai Shiba
Masaki Shirakawa
Masafumi Muratani
Takashi Kudo
Satoru Takahashi
author_sort Risa Okada
title Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
title_short Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
title_full Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
title_fullStr Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
title_full_unstemmed Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
title_sort transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/70a0d1618d87416e809859faa1469440
work_keys_str_mv AT risaokada transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT shinichirofujita transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT rikusuzuki transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT takutohayashi transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT hironatsubouchi transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT chihirokato transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT shunyasadaki transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT mahokanai transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT sayakafuseya transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT yuriinoue transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT hyojungjeon transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT michitohamada transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT akihirokuno transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT akikoishii transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT akiratamaoka transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT juntanihata transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT naokiito transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT daishiba transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT masakishirakawa transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT masafumimuratani transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT takashikudo transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
AT satorutakahashi transcriptomeanalysisofgravitationaleffectsonmouseskeletalmusclesundermicrogravityandartificial1gonboardenvironment
_version_ 1718392623189721088