Nano-antenna enhanced two-focus fluorescence correlation spectroscopy
Abstract We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that...
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Nature Portfolio
2017
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oai:doaj.org-article:70a3197eb03d4852994c128b20f0ce8b2021-12-02T11:52:15ZNano-antenna enhanced two-focus fluorescence correlation spectroscopy10.1038/s41598-017-06325-62045-2322https://doaj.org/article/70a3197eb03d4852994c128b20f0ce8b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06325-6https://doaj.org/toc/2045-2322Abstract We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that a calibrated distance between foci provides an intrinsic calibration to derive diffusion constants from measured correlation times. Through electromagnetic modelling we analyze a geometry of perpendicular nanorods, and their inverse, i.e., nanoslits. While we find that nanorods are not suited for nano-antenna enhanced 2fFCS due to substantial background signal, a nanoslit geometry is expected to provide a di tinct cross-correlation between orthogonally polarized detection channels. Furthermore, by utilizing a periodic array of nanoslits instead of a single pair, the amplitude of the cross-correlation can be enhanced. To demonstrate this technique, we present a proof of principle experiment on the basis of a periodic array of nanoslits, applied to lipid diffusion in a supported lipid bilayer.Lutz LangguthAgata SzubaSander A. MannErik C. GarnettGijsje H. KoenderinkA. Femius KoenderinkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
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Medicine R Science Q Lutz Langguth Agata Szuba Sander A. Mann Erik C. Garnett Gijsje H. Koenderink A. Femius Koenderink Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
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Abstract We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that a calibrated distance between foci provides an intrinsic calibration to derive diffusion constants from measured correlation times. Through electromagnetic modelling we analyze a geometry of perpendicular nanorods, and their inverse, i.e., nanoslits. While we find that nanorods are not suited for nano-antenna enhanced 2fFCS due to substantial background signal, a nanoslit geometry is expected to provide a di tinct cross-correlation between orthogonally polarized detection channels. Furthermore, by utilizing a periodic array of nanoslits instead of a single pair, the amplitude of the cross-correlation can be enhanced. To demonstrate this technique, we present a proof of principle experiment on the basis of a periodic array of nanoslits, applied to lipid diffusion in a supported lipid bilayer. |
format |
article |
author |
Lutz Langguth Agata Szuba Sander A. Mann Erik C. Garnett Gijsje H. Koenderink A. Femius Koenderink |
author_facet |
Lutz Langguth Agata Szuba Sander A. Mann Erik C. Garnett Gijsje H. Koenderink A. Femius Koenderink |
author_sort |
Lutz Langguth |
title |
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
title_short |
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
title_full |
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
title_fullStr |
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
title_full_unstemmed |
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
title_sort |
nano-antenna enhanced two-focus fluorescence correlation spectroscopy |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/70a3197eb03d4852994c128b20f0ce8b |
work_keys_str_mv |
AT lutzlangguth nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy AT agataszuba nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy AT sanderamann nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy AT erikcgarnett nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy AT gijsjehkoenderink nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy AT afemiuskoenderink nanoantennaenhancedtwofocusfluorescencecorrelationspectroscopy |
_version_ |
1718395109488197632 |