DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

Abstract We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon s...

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Autores principales:	Luiz Henrique Rosa, Thamar Holanda da Silva, Mayara Baptistucci Ogaki, Otávio Henrique Bezerra Pinto, Michael Stech, Peter Convey, Micheline Carvalho-Silva, Carlos Augusto Rosa, Paulo E. A. S. Câmara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70a51946cedd42ce8446eaf0a827a490
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spelling	oai:doaj.org-article:70a51946cedd42ce8446eaf0a827a4902021-12-02T11:57:57ZDNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica10.1038/s41598-020-78934-72045-2322https://doaj.org/article/70a51946cedd42ce8446eaf0a827a4902020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78934-7https://doaj.org/toc/2045-2322Abstract We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. We also detected taxa belonging to the rare phyla Mucoromycota and Rozellomycota, which have been difficult to detect in Antarctica by traditional isolation methods. Cladosporium sp., Pseudogymnoascus roseus, Leotiomycetes sp. 2, Penicillium sp., Mortierella sp. 1, Mortierella sp. 2, Pseudogymnoascus appendiculatus and Pseudogymnoascus sp. were the most dominant fungi. In addition, 440,153 of the total of 1,214,875 reads detected could be classified only at the level of Fungi. In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. Further long-term studies are required to elucidate how biological colonization in the island may be affected by climatic changes and/or other anthropogenic influences.Luiz Henrique RosaThamar Holanda da SilvaMayara Baptistucci OgakiOtávio Henrique Bezerra PintoMichael StechPeter ConveyMicheline Carvalho-SilvaCarlos Augusto RosaPaulo E. A. S. CâmaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luiz Henrique Rosa Thamar Holanda da Silva Mayara Baptistucci Ogaki Otávio Henrique Bezerra Pinto Michael Stech Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
description	Abstract We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. We also detected taxa belonging to the rare phyla Mucoromycota and Rozellomycota, which have been difficult to detect in Antarctica by traditional isolation methods. Cladosporium sp., Pseudogymnoascus roseus, Leotiomycetes sp. 2, Penicillium sp., Mortierella sp. 1, Mortierella sp. 2, Pseudogymnoascus appendiculatus and Pseudogymnoascus sp. were the most dominant fungi. In addition, 440,153 of the total of 1,214,875 reads detected could be classified only at the level of Fungi. In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. Further long-term studies are required to elucidate how biological colonization in the island may be affected by climatic changes and/or other anthropogenic influences.
format	article
author	Luiz Henrique Rosa Thamar Holanda da Silva Mayara Baptistucci Ogaki Otávio Henrique Bezerra Pinto Michael Stech Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara
author_facet	Luiz Henrique Rosa Thamar Holanda da Silva Mayara Baptistucci Ogaki Otávio Henrique Bezerra Pinto Michael Stech Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara
author_sort	Luiz Henrique Rosa
title	DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
title_short	DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
title_full	DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
title_fullStr	DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
title_full_unstemmed	DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
title_sort	dna metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on deception island, antarctica
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/70a51946cedd42ce8446eaf0a827a490
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DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

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