An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples

Introduction: Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay intended for measuring proteins and antibodies. (1) The ELISA method is tedious and a lengthy process. (2) HemosIL AcuStar VWF:CB is a fully automated method. Several publications have described a comparison between an EL...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sunny Jamati, Mark Paca
Formato:	article
Lenguaje:	EN RU UZ
Publicado:	Open Science LLC 2021
Materias:	automation chemiluminescent assay collagen binding activity elisa von willebrand disease Science (General) Q1-390 Education (General) L7-991
Acceso en línea:	https://doaj.org/article/70ab7c99823c4fb197577671dd4e62d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70ab7c99823c4fb197577671dd4e62d7
record_format	dspace
spelling	oai:doaj.org-article:70ab7c99823c4fb197577671dd4e62d72021-11-29T17:34:25ZAn assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples2181-0842https://doaj.org/article/70ab7c99823c4fb197577671dd4e62d72021-11-01T00:00:00Zhttps://openscience.uz/index.php/sciedu/article/view/1997https://doaj.org/toc/2181-0842Introduction: Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay intended for measuring proteins and antibodies. (1) The ELISA method is tedious and a lengthy process. (2) HemosIL AcuStar VWF:CB is a fully automated method. Several publications have described a comparison between an ELISA method and an automated method. A comparison was performed to evaluate the automated method. Method: A comparison between TECHNOZYM ® vWF:CBA ELISA and HemosIL AcuStar VWF:CB was performed. A total of 44 samples was included in the comparison which included congenital and acquired vWD. A reference interval was established; 50 healthy donors were collected from New Zealand Blood Service and from the laboratory and run on the and HemosIL AcuStar VWF:CB. Results: The HemosIL AcuStar is statistically analysed by Pearson’s Correlation coefficient (r2 ) and it showed a strong linear relationship (r=0.9795) with a negative bias of -14.6. The p-value was 0.0722 (>0.05) which is not statistically significant showing a strong evidence that even with a small sample size, the Hemosil AcuStar is a suitable replacement for Technozyme ELISA method. Conclusions: The statistical analysis demonstrated a good correlation between Acustar VWF:CB and ELISA assays for normal and VWD patients. HemosIL AcuStar VWF:CB is a potential alternative to a ELISA method for measuring CBA levels.Sunny JamatiMark PacaOpen Science LLCarticleautomationchemiluminescent assaycollagen binding activityelisavon willebrand diseaseScience (General)Q1-390Education (General)L7-991ENRUUZScience and Education, Vol 2, Iss 11, Pp 245-256 (2021)
institution	DOAJ
collection	DOAJ
language	EN RU UZ
topic	automation chemiluminescent assay collagen binding activity elisa von willebrand disease Science (General) Q1-390 Education (General) L7-991
spellingShingle	automation chemiluminescent assay collagen binding activity elisa von willebrand disease Science (General) Q1-390 Education (General) L7-991 Sunny Jamati Mark Paca An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
description	Introduction: Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay intended for measuring proteins and antibodies. (1) The ELISA method is tedious and a lengthy process. (2) HemosIL AcuStar VWF:CB is a fully automated method. Several publications have described a comparison between an ELISA method and an automated method. A comparison was performed to evaluate the automated method. Method: A comparison between TECHNOZYM ® vWF:CBA ELISA and HemosIL AcuStar VWF:CB was performed. A total of 44 samples was included in the comparison which included congenital and acquired vWD. A reference interval was established; 50 healthy donors were collected from New Zealand Blood Service and from the laboratory and run on the and HemosIL AcuStar VWF:CB. Results: The HemosIL AcuStar is statistically analysed by Pearson’s Correlation coefficient (r2 ) and it showed a strong linear relationship (r=0.9795) with a negative bias of -14.6. The p-value was 0.0722 (>0.05) which is not statistically significant showing a strong evidence that even with a small sample size, the Hemosil AcuStar is a suitable replacement for Technozyme ELISA method. Conclusions: The statistical analysis demonstrated a good correlation between Acustar VWF:CB and ELISA assays for normal and VWD patients. HemosIL AcuStar VWF:CB is a potential alternative to a ELISA method for measuring CBA levels.
format	article
author	Sunny Jamati Mark Paca
author_facet	Sunny Jamati Mark Paca
author_sort	Sunny Jamati
title	An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
title_short	An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
title_full	An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
title_fullStr	An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
title_full_unstemmed	An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples
title_sort	assessment of an automated assay and an elisa method for collagen binding activity levels using congenital and acquired von willebrand disease samples
publisher	Open Science LLC
publishDate	2021
url	https://doaj.org/article/70ab7c99823c4fb197577671dd4e62d7
work_keys_str_mv	AT sunnyjamati anassessmentofanautomatedassayandanelisamethodforcollagenbindingactivitylevelsusingcongenitalandacquiredvonwillebranddiseasesamples AT markpaca anassessmentofanautomatedassayandanelisamethodforcollagenbindingactivitylevelsusingcongenitalandacquiredvonwillebranddiseasesamples AT sunnyjamati assessmentofanautomatedassayandanelisamethodforcollagenbindingactivitylevelsusingcongenitalandacquiredvonwillebranddiseasesamples AT markpaca assessmentofanautomatedassayandanelisamethodforcollagenbindingactivitylevelsusingcongenitalandacquiredvonwillebranddiseasesamples
_version_	1718407167950716928

An assessment of an automated assay and an ELISA method for collagen binding activity levels using congenital and acquired von Willebrand disease samples

Ejemplares similares