Inducible and tunable gene expression systems for Pseudomonas putida KT2440

Abstract Inducible and tunable expression systems are essential for the microbial production of biochemicals. Five different carbon source- and substrate-inducible promoter systems were developed and further evaluated in Pseudomonas putida KT2440 by analyzing the expression of green fluorescent prot...

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Autores principales:	Chandran Sathesh-Prabu, Rameshwar Tiwari, Doyun Kim, Sung Kuk Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70b05f10c78f4ed5a04ddce7579e19e1
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spelling	oai:doaj.org-article:70b05f10c78f4ed5a04ddce7579e19e12021-12-02T14:58:47ZInducible and tunable gene expression systems for Pseudomonas putida KT244010.1038/s41598-021-97550-72045-2322https://doaj.org/article/70b05f10c78f4ed5a04ddce7579e19e12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97550-7https://doaj.org/toc/2045-2322Abstract Inducible and tunable expression systems are essential for the microbial production of biochemicals. Five different carbon source- and substrate-inducible promoter systems were developed and further evaluated in Pseudomonas putida KT2440 by analyzing the expression of green fluorescent protein (GFP) as a reporter protein. These systems can be induced by low-cost compounds such as glucose, 3-hydroxypropionic acid (3HP), levulinic acid (LA), and xylose. 3HP-inducible HpdR/P hpdH was also efficiently induced by LA. LvaR/P lvaA and XutR/P xutA systems were induced even at low concentrations of LA (0.1 mM) and xylose (0.5 mM), respectively. Glucose-inducible HexR/P zwf1 showed weak GFP expression. These inducer agents can be used as potent starting materials for both cell growth and the production of a wide range of biochemicals. The efficiency of the reported systems was comparable to that of conventional chemical-inducible systems. Hence, the newly investigated promoter systems are highly useful for the expression of target genes in the widely used synthetic biology chassis P. putida KT2440 for industrial and medical applications.Chandran Sathesh-PrabuRameshwar TiwariDoyun KimSung Kuk LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chandran Sathesh-Prabu Rameshwar Tiwari Doyun Kim Sung Kuk Lee Inducible and tunable gene expression systems for Pseudomonas putida KT2440
description	Abstract Inducible and tunable expression systems are essential for the microbial production of biochemicals. Five different carbon source- and substrate-inducible promoter systems were developed and further evaluated in Pseudomonas putida KT2440 by analyzing the expression of green fluorescent protein (GFP) as a reporter protein. These systems can be induced by low-cost compounds such as glucose, 3-hydroxypropionic acid (3HP), levulinic acid (LA), and xylose. 3HP-inducible HpdR/P hpdH was also efficiently induced by LA. LvaR/P lvaA and XutR/P xutA systems were induced even at low concentrations of LA (0.1 mM) and xylose (0.5 mM), respectively. Glucose-inducible HexR/P zwf1 showed weak GFP expression. These inducer agents can be used as potent starting materials for both cell growth and the production of a wide range of biochemicals. The efficiency of the reported systems was comparable to that of conventional chemical-inducible systems. Hence, the newly investigated promoter systems are highly useful for the expression of target genes in the widely used synthetic biology chassis P. putida KT2440 for industrial and medical applications.
format	article
author	Chandran Sathesh-Prabu Rameshwar Tiwari Doyun Kim Sung Kuk Lee
author_facet	Chandran Sathesh-Prabu Rameshwar Tiwari Doyun Kim Sung Kuk Lee
author_sort	Chandran Sathesh-Prabu
title	Inducible and tunable gene expression systems for Pseudomonas putida KT2440
title_short	Inducible and tunable gene expression systems for Pseudomonas putida KT2440
title_full	Inducible and tunable gene expression systems for Pseudomonas putida KT2440
title_fullStr	Inducible and tunable gene expression systems for Pseudomonas putida KT2440
title_full_unstemmed	Inducible and tunable gene expression systems for Pseudomonas putida KT2440
title_sort	inducible and tunable gene expression systems for pseudomonas putida kt2440
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70b05f10c78f4ed5a04ddce7579e19e1
work_keys_str_mv	AT chandransatheshprabu inducibleandtunablegeneexpressionsystemsforpseudomonasputidakt2440 AT rameshwartiwari inducibleandtunablegeneexpressionsystemsforpseudomonasputidakt2440 AT doyunkim inducibleandtunablegeneexpressionsystemsforpseudomonasputidakt2440 AT sungkuklee inducibleandtunablegeneexpressionsystemsforpseudomonasputidakt2440
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Inducible and tunable gene expression systems for Pseudomonas putida KT2440

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