A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury
Abstract NMDA receptor (NMDAR) is known for its ionotropic function. But recent evidence suggests that NMDAR also has a non-ionotropic property. To determine the role of non-ionotropic activity of NMDARs in clinical relevant conditions, we tested the effect of glycine, a co-agonist of NMDARs, in rat...
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Nature Portfolio
2017
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oai:doaj.org-article:70b56126228b48c491e3480be28700cd2021-12-02T15:04:57ZA non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury10.1038/s41598-017-03909-02045-2322https://doaj.org/article/70b56126228b48c491e3480be28700cd2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03909-0https://doaj.org/toc/2045-2322Abstract NMDA receptor (NMDAR) is known for its ionotropic function. But recent evidence suggests that NMDAR also has a non-ionotropic property. To determine the role of non-ionotropic activity of NMDARs in clinical relevant conditions, we tested the effect of glycine, a co-agonist of NMDARs, in rat middle cerebral artery occlusion (MCAO), an animal model of cerebral ischemia-reperfusion injury after the animals were injected with the NMDAR channel blocker MK-801 and the glycine receptor antagonist strychnine. We show that glycine reduces the infarct volume in the brain of ischemic stroke animals pre-injected with MK-801 and strychnine. The effect of glycine is sensitive to the antagonist of glycine-GluN1 binding site and blocked by Akt inhibition. In the neurobehavioral tests, glycine improves the functional recovery of stroke animals pre-injected with MK-801 and strychnine. This study suggests that glycine-induced neuroprotection is mediated in part by the non-ionotropic activity of NMDARs via Akt activation in cerebral ischemia-reperfusion injury.Juan ChenRong HuHuabao LiaoYa ZhangRuixue LeiZhifeng ZhangYang ZhuangYu WanPing JinHua FengQi WanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Juan Chen Rong Hu Huabao Liao Ya Zhang Ruixue Lei Zhifeng Zhang Yang Zhuang Yu Wan Ping Jin Hua Feng Qi Wan A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| description |
Abstract NMDA receptor (NMDAR) is known for its ionotropic function. But recent evidence suggests that NMDAR also has a non-ionotropic property. To determine the role of non-ionotropic activity of NMDARs in clinical relevant conditions, we tested the effect of glycine, a co-agonist of NMDARs, in rat middle cerebral artery occlusion (MCAO), an animal model of cerebral ischemia-reperfusion injury after the animals were injected with the NMDAR channel blocker MK-801 and the glycine receptor antagonist strychnine. We show that glycine reduces the infarct volume in the brain of ischemic stroke animals pre-injected with MK-801 and strychnine. The effect of glycine is sensitive to the antagonist of glycine-GluN1 binding site and blocked by Akt inhibition. In the neurobehavioral tests, glycine improves the functional recovery of stroke animals pre-injected with MK-801 and strychnine. This study suggests that glycine-induced neuroprotection is mediated in part by the non-ionotropic activity of NMDARs via Akt activation in cerebral ischemia-reperfusion injury. |
| format |
article |
| author |
Juan Chen Rong Hu Huabao Liao Ya Zhang Ruixue Lei Zhifeng Zhang Yang Zhuang Yu Wan Ping Jin Hua Feng Qi Wan |
| author_facet |
Juan Chen Rong Hu Huabao Liao Ya Zhang Ruixue Lei Zhifeng Zhang Yang Zhuang Yu Wan Ping Jin Hua Feng Qi Wan |
| author_sort |
Juan Chen |
| title |
A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| title_short |
A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| title_full |
A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| title_fullStr |
A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| title_full_unstemmed |
A non-ionotropic activity of NMDA receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| title_sort |
non-ionotropic activity of nmda receptors contributes to glycine-induced neuroprotection in cerebral ischemia-reperfusion injury |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/70b56126228b48c491e3480be28700cd |
| work_keys_str_mv |
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