Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening

Abstract Endothelial damage and fibro-proliferative vasculopathy of small vessels are pathological hallmarks of systemic sclerosis (SSc). The consequence is the detachment of resident elements that become circulating endothelial cells (CECs). The aim of our study was to evaluate the potential of CEC...

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Autores principales:	Maria Luisa Di Martino, Alessandra Frau, Francesca Losa, Emma Muggianu, Mario Nicola Mura, Gianluca Rotta, Lorenza Scotti, Francesco Marongiu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70c6d22f97e94afba984218cc59d67cc
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spelling	oai:doaj.org-article:70c6d22f97e94afba984218cc59d67cc2021-12-02T10:47:54ZRole of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening10.1038/s41598-020-80604-72045-2322https://doaj.org/article/70c6d22f97e94afba984218cc59d67cc2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80604-7https://doaj.org/toc/2045-2322Abstract Endothelial damage and fibro-proliferative vasculopathy of small vessels are pathological hallmarks of systemic sclerosis (SSc). The consequence is the detachment of resident elements that become circulating endothelial cells (CECs). The aim of our study was to evaluate the potential of CECs as biomarker in SSc. We enrolled 50 patients with limited cutaneous (lcSSc) and diffuse cutaneous (dcSSc) subset of SSc, who underwent clinical evaluation to establish the organ involvement. CECs were measured by flow-cytometry utilizing a polychromatic panel. An evident difference was observed in CEC counts comparing controls to SSc patients (median 10.5 vs. 152 cells/ml, p < 0.0001) and for the first time, between the two subsets of disease (median lcSSc 132 vs. dcSSc 716 CEC/ml, p < 0.0001). A significant correlation was established between CECs and some SSc clinical parameters, such as digital ulcers, skin and pulmonary involvement, presence of Scl-70 antibodies, nailfold videocapillaroscopy patterns and EUSTAR activity index. After 12 months, CECs correlated with clinical worsening of patients, showing that a number higher than 414 CEC/ml is a strong negative prognostic factor (RR 5.70). Our results indicate that CECs are a direct indicator of systemic vascular damage. Therefore, they can be used as a reliable marker of disease severity.Maria Luisa Di MartinoAlessandra FrauFrancesca LosaEmma MuggianuMario Nicola MuraGianluca RottaLorenza ScottiFrancesco MarongiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Luisa Di Martino Alessandra Frau Francesca Losa Emma Muggianu Mario Nicola Mura Gianluca Rotta Lorenza Scotti Francesco Marongiu Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
description	Abstract Endothelial damage and fibro-proliferative vasculopathy of small vessels are pathological hallmarks of systemic sclerosis (SSc). The consequence is the detachment of resident elements that become circulating endothelial cells (CECs). The aim of our study was to evaluate the potential of CECs as biomarker in SSc. We enrolled 50 patients with limited cutaneous (lcSSc) and diffuse cutaneous (dcSSc) subset of SSc, who underwent clinical evaluation to establish the organ involvement. CECs were measured by flow-cytometry utilizing a polychromatic panel. An evident difference was observed in CEC counts comparing controls to SSc patients (median 10.5 vs. 152 cells/ml, p < 0.0001) and for the first time, between the two subsets of disease (median lcSSc 132 vs. dcSSc 716 CEC/ml, p < 0.0001). A significant correlation was established between CECs and some SSc clinical parameters, such as digital ulcers, skin and pulmonary involvement, presence of Scl-70 antibodies, nailfold videocapillaroscopy patterns and EUSTAR activity index. After 12 months, CECs correlated with clinical worsening of patients, showing that a number higher than 414 CEC/ml is a strong negative prognostic factor (RR 5.70). Our results indicate that CECs are a direct indicator of systemic vascular damage. Therefore, they can be used as a reliable marker of disease severity.
format	article
author	Maria Luisa Di Martino Alessandra Frau Francesca Losa Emma Muggianu Mario Nicola Mura Gianluca Rotta Lorenza Scotti Francesco Marongiu
author_facet	Maria Luisa Di Martino Alessandra Frau Francesca Losa Emma Muggianu Mario Nicola Mura Gianluca Rotta Lorenza Scotti Francesco Marongiu
author_sort	Maria Luisa Di Martino
title	Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
title_short	Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
title_full	Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
title_fullStr	Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
title_full_unstemmed	Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
title_sort	role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70c6d22f97e94afba984218cc59d67cc
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Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening

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