PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression

Gastric cancer is the third leading cause of cancer death worldwide. Traditional Chinese medicine (TCM) is increasingly extensively applied as a complementary therapy for gastric cancer (GC) in China, which shows unique advantages in preventing gastric cancer metastasis. Previous study indicates mod...

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Autores principales:	Qingmin Sun, Mengyun Yuan, Hongxing Wang, Xingxing Zhang, Ruijuan Zhang, Haidan Wang, Xu Chen, Min Zhu, Shenlin Liu, Jian Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	traditional Chinese medicine PKM2 gastric cancer aerobic glycolysis cancer metabolism Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/70cf44ee81a641ff9899aba70027aa18
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spelling	oai:doaj.org-article:70cf44ee81a641ff9899aba70027aa182021-12-02T11:06:52ZPKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression2234-943X10.3389/fonc.2021.767116https://doaj.org/article/70cf44ee81a641ff9899aba70027aa182021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.767116/fullhttps://doaj.org/toc/2234-943XGastric cancer is the third leading cause of cancer death worldwide. Traditional Chinese medicine (TCM) is increasingly extensively applied as a complementary therapy for gastric cancer (GC) in China, which shows unique advantages in preventing gastric cancer metastasis. Previous study indicates modified Jian-pi-yang-zheng (mJPYZ) decoction inhibit the progression of gastric cancer by regulating tumor-associated macrophages (TAM). However, it is unclear whether mJPYZ can affect metabolic reprogramming of gastric cancer cells. Here, we showed that mJPYZ effectively attenuated GC cells proliferation, migration and invasion. Meantime, mJPYZ reduced the aerobic glycolysis level of GC cells in vivo and in vitro by regulating the expression and nuclear translocation of PKM2. Overexpression of PKM2 that could reverse the inhibitory effect of mJPYZ, migration and epithelial to mesenchymal transition (EMT). Our results showed PKM2/HIF-1α signaling was the key metabolic regulator of mJPYZ in GC cells. In summary, our present study suggested that abnormal PKM2 is required for maintaining the malignant phenotype of GC cells. The TCM decoction mJPYZ inhibited GC cells growth and EMT by reducing of glycolysis in PKM2 dependent manner. This evidence expanded our understanding of the anti-tumor mechanism of mJPYZ and further indicated mJPYZ a potential anti-tumor agent for GC patients.Chemical Compounds Studied in this ArticleRutin (PubChem CID: 5280805); Lobetyolin (PubChem CID: 53486204); Calycosin-7-glucoside (PubChem CID: 71571502); Formononetin (PubChem CID: 5280378); Calycosin (PubChem CID: 5280448); Ononin (PubChem CID: 442813); P-Coumaric Acid (PubChem CID: 637542).Qingmin SunMengyun YuanMengyun YuanHongxing WangHongxing WangXingxing ZhangRuijuan ZhangRuijuan ZhangHaidan WangXu ChenXu ChenMin ZhuShenlin LiuJian WuFrontiers Media S.A.articletraditional Chinese medicinePKM2gastric canceraerobic glycolysiscancer metabolismNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	traditional Chinese medicine PKM2 gastric cancer aerobic glycolysis cancer metabolism Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	traditional Chinese medicine PKM2 gastric cancer aerobic glycolysis cancer metabolism Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Qingmin Sun Mengyun Yuan Mengyun Yuan Hongxing Wang Hongxing Wang Xingxing Zhang Ruijuan Zhang Ruijuan Zhang Haidan Wang Xu Chen Xu Chen Min Zhu Shenlin Liu Jian Wu PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
description	Gastric cancer is the third leading cause of cancer death worldwide. Traditional Chinese medicine (TCM) is increasingly extensively applied as a complementary therapy for gastric cancer (GC) in China, which shows unique advantages in preventing gastric cancer metastasis. Previous study indicates modified Jian-pi-yang-zheng (mJPYZ) decoction inhibit the progression of gastric cancer by regulating tumor-associated macrophages (TAM). However, it is unclear whether mJPYZ can affect metabolic reprogramming of gastric cancer cells. Here, we showed that mJPYZ effectively attenuated GC cells proliferation, migration and invasion. Meantime, mJPYZ reduced the aerobic glycolysis level of GC cells in vivo and in vitro by regulating the expression and nuclear translocation of PKM2. Overexpression of PKM2 that could reverse the inhibitory effect of mJPYZ, migration and epithelial to mesenchymal transition (EMT). Our results showed PKM2/HIF-1α signaling was the key metabolic regulator of mJPYZ in GC cells. In summary, our present study suggested that abnormal PKM2 is required for maintaining the malignant phenotype of GC cells. The TCM decoction mJPYZ inhibited GC cells growth and EMT by reducing of glycolysis in PKM2 dependent manner. This evidence expanded our understanding of the anti-tumor mechanism of mJPYZ and further indicated mJPYZ a potential anti-tumor agent for GC patients.Chemical Compounds Studied in this ArticleRutin (PubChem CID: 5280805); Lobetyolin (PubChem CID: 53486204); Calycosin-7-glucoside (PubChem CID: 71571502); Formononetin (PubChem CID: 5280378); Calycosin (PubChem CID: 5280448); Ononin (PubChem CID: 442813); P-Coumaric Acid (PubChem CID: 637542).
format	article
author	Qingmin Sun Mengyun Yuan Mengyun Yuan Hongxing Wang Hongxing Wang Xingxing Zhang Ruijuan Zhang Ruijuan Zhang Haidan Wang Xu Chen Xu Chen Min Zhu Shenlin Liu Jian Wu
author_facet	Qingmin Sun Mengyun Yuan Mengyun Yuan Hongxing Wang Hongxing Wang Xingxing Zhang Ruijuan Zhang Ruijuan Zhang Haidan Wang Xu Chen Xu Chen Min Zhu Shenlin Liu Jian Wu
author_sort	Qingmin Sun
title	PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
title_short	PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
title_full	PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
title_fullStr	PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
title_full_unstemmed	PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression
title_sort	pkm2 is the target of a multi-herb-combined decoction during the inhibition of gastric cancer progression
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/70cf44ee81a641ff9899aba70027aa18
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PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression

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