Metabolite Profiling of Helichrysum italicum Derived Food Supplements by 1H-NMR-Based Metabolomics

Helichrysum italicum is a medicinal plant from the Mediterranean area, widely used in traditional medicine for its anti-inflammatory, antibacterial and antioxidant properties and for its preventive effects on microcirculation diseases. Due to these properties, it finds large appli...

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Autores principales:	Antonietta Cerulli, Milena Masullo, Sonia Piacente
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	green extractions <i>Helichrysum italicum</i> derived food supplements NMR metabolomics multivariate data analysis Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/70cf4605e4f7468c8a360ee6b75173cd
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id	oai:doaj.org-article:70cf4605e4f7468c8a360ee6b75173cd
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spelling	oai:doaj.org-article:70cf4605e4f7468c8a360ee6b75173cd2021-11-11T18:35:31ZMetabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics10.3390/molecules262166191420-3049https://doaj.org/article/70cf4605e4f7468c8a360ee6b75173cd2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6619https://doaj.org/toc/1420-3049<i>Helichrysum italicum</i> is a medicinal plant from the Mediterranean area, widely used in traditional medicine for its anti-inflammatory, antibacterial and antioxidant properties and for its preventive effects on microcirculation diseases. Due to these properties, it finds large applications in cosmetic, food and pharmaceutical fields. Additionally, hydroalcoholic extracts and mother tinctures based on <i>H. italicum</i> represent products with a high commercial value, widely distributed not only in drug stores but also on on-line markets. The different extraction procedures used can greatly affect the fingerprints of the extracts, resulting in a different qualitative or quantitative profile of the chemical constituents responsible for biological activity. The aim of the present study was to characterize the composition of bioactive compounds present in water-ethanol and glycerol extracts of <i>H. italicum</i> derived food supplements. Metabolite profiles of the extracts were obtained by <sup>1</sup>H NMR experiments and data were processed by multivariate statistical analysis to highlight differences in the extracts and to evidence the extracts with the highest concentrations of bioactive metabolites. In detail, this work highlights how derived food supplements of <i>H. italicum</i> obtained using ethanol-water mixtures ranging from 45% to 20% of ethanol represent the products with the highest amount of both primary (amino acids) and secondary metabolites including 3,4-dicaffeoylquinic acid (<b>9</b>), chlorogenic acid (<b>10</b>), 3,5-dicaffeoylquinic acid (<b>11</b>), and kaempferol 3-<i>O</i>-glucopyranoside (<b>12</b>). Moreover, it is evident that the use of an ethanol-water mixture 20:80 is the most suitable method to afford the highest number of phenolic compounds, while food-derived supplements obtained by glycerol extraction are characterized by a high amount of β-glucose and α-glucose and a low content of phenolic compounds.Antonietta CerulliMilena MasulloSonia PiacenteMDPI AGarticlegreen extractions<i>Helichrysum italicum</i> derived food supplementsNMR metabolomicsmultivariate data analysisOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6619, p 6619 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	green extractions <i>Helichrysum italicum</i> derived food supplements NMR metabolomics multivariate data analysis Organic chemistry QD241-441
spellingShingle	green extractions <i>Helichrysum italicum</i> derived food supplements NMR metabolomics multivariate data analysis Organic chemistry QD241-441 Antonietta Cerulli Milena Masullo Sonia Piacente Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
description	<i>Helichrysum italicum</i> is a medicinal plant from the Mediterranean area, widely used in traditional medicine for its anti-inflammatory, antibacterial and antioxidant properties and for its preventive effects on microcirculation diseases. Due to these properties, it finds large applications in cosmetic, food and pharmaceutical fields. Additionally, hydroalcoholic extracts and mother tinctures based on <i>H. italicum</i> represent products with a high commercial value, widely distributed not only in drug stores but also on on-line markets. The different extraction procedures used can greatly affect the fingerprints of the extracts, resulting in a different qualitative or quantitative profile of the chemical constituents responsible for biological activity. The aim of the present study was to characterize the composition of bioactive compounds present in water-ethanol and glycerol extracts of <i>H. italicum</i> derived food supplements. Metabolite profiles of the extracts were obtained by <sup>1</sup>H NMR experiments and data were processed by multivariate statistical analysis to highlight differences in the extracts and to evidence the extracts with the highest concentrations of bioactive metabolites. In detail, this work highlights how derived food supplements of <i>H. italicum</i> obtained using ethanol-water mixtures ranging from 45% to 20% of ethanol represent the products with the highest amount of both primary (amino acids) and secondary metabolites including 3,4-dicaffeoylquinic acid (<b>9</b>), chlorogenic acid (<b>10</b>), 3,5-dicaffeoylquinic acid (<b>11</b>), and kaempferol 3-<i>O</i>-glucopyranoside (<b>12</b>). Moreover, it is evident that the use of an ethanol-water mixture 20:80 is the most suitable method to afford the highest number of phenolic compounds, while food-derived supplements obtained by glycerol extraction are characterized by a high amount of β-glucose and α-glucose and a low content of phenolic compounds.
format	article
author	Antonietta Cerulli Milena Masullo Sonia Piacente
author_facet	Antonietta Cerulli Milena Masullo Sonia Piacente
author_sort	Antonietta Cerulli
title	Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
title_short	Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
title_full	Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
title_fullStr	Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
title_full_unstemmed	Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics
title_sort	metabolite profiling of <i>helichrysum italicum</i> derived food supplements by <sup>1</sup>h-nmr-based metabolomics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/70cf4605e4f7468c8a360ee6b75173cd
work_keys_str_mv	AT antoniettacerulli metaboliteprofilingofihelichrysumitalicumiderivedfoodsupplementsbysup1suphnmrbasedmetabolomics AT milenamasullo metaboliteprofilingofihelichrysumitalicumiderivedfoodsupplementsbysup1suphnmrbasedmetabolomics AT soniapiacente metaboliteprofilingofihelichrysumitalicumiderivedfoodsupplementsbysup1suphnmrbasedmetabolomics
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Metabolite Profiling of <i>Helichrysum italicum</i> Derived Food Supplements by <sup>1</sup>H-NMR-Based Metabolomics

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