Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.

The immune system relies on the plasticity of its components to produce appropriate responses to frequent environmental challenges. Dendritic cells (DCs) are critical initiators of innate immunity and orchestrate the later and more specific adaptive immunity. The generation of diversity in transcrip...

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Autores principales:	Raquel Rodrigues, Ana Rita Grosso, Luís Moita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70d1c7c36be5456799ad7c1821a7713a
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spelling	oai:doaj.org-article:70d1c7c36be5456799ad7c1821a7713a2021-11-18T07:48:58ZGenome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.1932-620310.1371/journal.pone.0061975https://doaj.org/article/70d1c7c36be5456799ad7c1821a7713a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613991/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The immune system relies on the plasticity of its components to produce appropriate responses to frequent environmental challenges. Dendritic cells (DCs) are critical initiators of innate immunity and orchestrate the later and more specific adaptive immunity. The generation of diversity in transcriptional programs is central for effective immune responses. Alternative splicing is widely considered a key generator of transcriptional and proteomic complexity, but its role has been rarely addressed systematically in immune cells. Here we used splicing-sensitive arrays to assess genome-wide gene- and exon-level expression profiles in human DCs in response to a bacterial challenge. We find widespread alternative splicing events and splicing factor transcriptional signatures induced by an E. coli challenge to human DCs. Alternative splicing acts in concert with transcriptional modulation, but these two mechanisms of gene regulation affect primarily distinct functional gene groups. Alternative splicing is likely to have an important role in DC immunobiology because it affects genes known to be involved in DC development, endocytosis, antigen presentation and cell cycle arrest.Raquel RodriguesAna Rita GrossoLuís MoitaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61975 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raquel Rodrigues Ana Rita Grosso Luís Moita Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
description	The immune system relies on the plasticity of its components to produce appropriate responses to frequent environmental challenges. Dendritic cells (DCs) are critical initiators of innate immunity and orchestrate the later and more specific adaptive immunity. The generation of diversity in transcriptional programs is central for effective immune responses. Alternative splicing is widely considered a key generator of transcriptional and proteomic complexity, but its role has been rarely addressed systematically in immune cells. Here we used splicing-sensitive arrays to assess genome-wide gene- and exon-level expression profiles in human DCs in response to a bacterial challenge. We find widespread alternative splicing events and splicing factor transcriptional signatures induced by an E. coli challenge to human DCs. Alternative splicing acts in concert with transcriptional modulation, but these two mechanisms of gene regulation affect primarily distinct functional gene groups. Alternative splicing is likely to have an important role in DC immunobiology because it affects genes known to be involved in DC development, endocytosis, antigen presentation and cell cycle arrest.
format	article
author	Raquel Rodrigues Ana Rita Grosso Luís Moita
author_facet	Raquel Rodrigues Ana Rita Grosso Luís Moita
author_sort	Raquel Rodrigues
title	Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
title_short	Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
title_full	Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
title_fullStr	Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
title_full_unstemmed	Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
title_sort	genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/70d1c7c36be5456799ad7c1821a7713a
work_keys_str_mv	AT raquelrodrigues genomewideanalysisofalternativesplicingduringdendriticcellresponsetoabacterialchallenge AT anaritagrosso genomewideanalysisofalternativesplicingduringdendriticcellresponsetoabacterialchallenge AT luismoita genomewideanalysisofalternativesplicingduringdendriticcellresponsetoabacterialchallenge
_version_	1718422917163778048

Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.

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