Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles

Background: There are different methods for genomic DNA extraction. Magnetic nanoparticles (MNPs) exhibit many important features making them suitable for DNA extraction. Objectives: The aim of this study was to compare the effect of monolayer and bilayer MNPs on genomic DNA extraction. Materials an...

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Autores principales:	Hadiseh Rostami, Farzaneh Firoozeh, Mohammad Zibaei, Iman Salahshoorifar
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	magnetic nanoparticles staphylococcus aureus genomic dna dna extraction Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/70d2498a87d443a6bb8a1a382f132887
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spelling	oai:doaj.org-article:70d2498a87d443a6bb8a1a382f1328872021-11-18T06:56:28ZEvaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles2345-33622322-586610.34172/ijep.2020.11https://doaj.org/article/70d2498a87d443a6bb8a1a382f1328872020-05-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4349https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: There are different methods for genomic DNA extraction. Magnetic nanoparticles (MNPs) exhibit many important features making them suitable for DNA extraction. Objectives: The aim of this study was to compare the effect of monolayer and bilayer MNPs on genomic DNA extraction. Materials and Methods: The genomic DNA was extracted from the Staphylococcus aureus strain ATCC 25923 and clinical isolates using monolayer MNPs SiO2 and Fe3O4 and bilayer MNPs SiO2/Fe3O4. Then, the quality and quantity of the obtained genomic DNA were compared with both NPs. Results: The obtained results showed the concentration and purity of the extracted genomic DNA using bilayer magnetic NPs was significantly higher in comparison to the extracted genomic DNA by monolayer MNPs. Conclusion: In general, surface-coated MNPs are much more efficient than naked MNPs for genomic DNA extraction.Hadiseh RostamiFarzaneh FiroozehMohammad ZibaeiIman SalahshoorifarAlborz University of Medical Sciencesarticlemagnetic nanoparticles staphylococcus aureus genomic dna dna extraction Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 2, Pp 51-54 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic nanoparticles staphylococcus aureus genomic dna dna extraction Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	magnetic nanoparticles staphylococcus aureus genomic dna dna extraction Diseases of the digestive system. Gastroenterology RC799-869 Hadiseh Rostami Farzaneh Firoozeh Mohammad Zibaei Iman Salahshoorifar Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
description	Background: There are different methods for genomic DNA extraction. Magnetic nanoparticles (MNPs) exhibit many important features making them suitable for DNA extraction. Objectives: The aim of this study was to compare the effect of monolayer and bilayer MNPs on genomic DNA extraction. Materials and Methods: The genomic DNA was extracted from the Staphylococcus aureus strain ATCC 25923 and clinical isolates using monolayer MNPs SiO2 and Fe3O4 and bilayer MNPs SiO2/Fe3O4. Then, the quality and quantity of the obtained genomic DNA were compared with both NPs. Results: The obtained results showed the concentration and purity of the extracted genomic DNA using bilayer magnetic NPs was significantly higher in comparison to the extracted genomic DNA by monolayer MNPs. Conclusion: In general, surface-coated MNPs are much more efficient than naked MNPs for genomic DNA extraction.
format	article
author	Hadiseh Rostami Farzaneh Firoozeh Mohammad Zibaei Iman Salahshoorifar
author_facet	Hadiseh Rostami Farzaneh Firoozeh Mohammad Zibaei Iman Salahshoorifar
author_sort	Hadiseh Rostami
title	Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
title_short	Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
title_full	Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
title_fullStr	Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
title_full_unstemmed	Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles
title_sort	evaluation of genomic dna extraction using monolayer and bilayer magnetic nanoparticles
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/70d2498a87d443a6bb8a1a382f132887
work_keys_str_mv	AT hadisehrostami evaluationofgenomicdnaextractionusingmonolayerandbilayermagneticnanoparticles AT farzanehfiroozeh evaluationofgenomicdnaextractionusingmonolayerandbilayermagneticnanoparticles AT mohammadzibaei evaluationofgenomicdnaextractionusingmonolayerandbilayermagneticnanoparticles AT imansalahshoorifar evaluationofgenomicdnaextractionusingmonolayerandbilayermagneticnanoparticles
_version_	1718424172237946880

Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles

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