Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress

Abstract In arid and semiarid regions, low precipitation rates lead to soil salinity problems, which may limit plant establishment, growth, and survival. Herein, we investigated the NaCl stress effect on chlorophyll fluorescence, photosynthetic-pigments, movement and chloroplasts ultrastructure in c...

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Autores principales:	Diana Marcela Arias-Moreno, Juan Francisco Jiménez-Bremont, Israel Maruri-López, Pablo Delgado-Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70d3cf5aa95547119a072d8cca9a4957
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spelling	oai:doaj.org-article:70d3cf5aa95547119a072d8cca9a49572021-12-02T15:06:15ZEffects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress10.1038/s41598-017-08744-x2045-2322https://doaj.org/article/70d3cf5aa95547119a072d8cca9a49572017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08744-xhttps://doaj.org/toc/2045-2322Abstract In arid and semiarid regions, low precipitation rates lead to soil salinity problems, which may limit plant establishment, growth, and survival. Herein, we investigated the NaCl stress effect on chlorophyll fluorescence, photosynthetic-pigments, movement and chloroplasts ultrastructure in chlorenchyma cells of Opuntia streptacantha cladodes. Cladodes segments were exposed to salt stress at 0, 100, 200, and 300 mM NaCl for 8, 16, and 24 h. The results showed that salt stress reduced chlorophyll content, F v /F m , ΦPSII, and qP values. Under the highest salt stress treatments, the chloroplasts were densely clumped toward the cell center and thylakoid membranes were notably affected. We analyzed the effect of exogenous catalase in salt-stressed cladode segments during 8, 16, and 24 h. The catalase application to salt-stressed cladodes counteracted the NaCl adverse effects, increasing the chlorophyll fluorescence parameters, photosynthetic-pigments, and avoided chloroplast clustering. Our results indicate that salt stress triggered the chloroplast clumping and affected the photosynthesis in O. streptacantha chlorenchyma cells. The exogenous catalase reverted the H2O2 accumulation and clustering of chloroplast, which led to an improvement of the photosynthetic efficiency. These data suggest that H2O2 detoxification by catalase is important to protect the chloroplast, thus conserving the photosynthetic activity in O. streptacantha under stress.Diana Marcela Arias-MorenoJuan Francisco Jiménez-BremontIsrael Maruri-LópezPablo Delgado-SánchezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diana Marcela Arias-Moreno Juan Francisco Jiménez-Bremont Israel Maruri-López Pablo Delgado-Sánchez Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
description	Abstract In arid and semiarid regions, low precipitation rates lead to soil salinity problems, which may limit plant establishment, growth, and survival. Herein, we investigated the NaCl stress effect on chlorophyll fluorescence, photosynthetic-pigments, movement and chloroplasts ultrastructure in chlorenchyma cells of Opuntia streptacantha cladodes. Cladodes segments were exposed to salt stress at 0, 100, 200, and 300 mM NaCl for 8, 16, and 24 h. The results showed that salt stress reduced chlorophyll content, F v /F m , ΦPSII, and qP values. Under the highest salt stress treatments, the chloroplasts were densely clumped toward the cell center and thylakoid membranes were notably affected. We analyzed the effect of exogenous catalase in salt-stressed cladode segments during 8, 16, and 24 h. The catalase application to salt-stressed cladodes counteracted the NaCl adverse effects, increasing the chlorophyll fluorescence parameters, photosynthetic-pigments, and avoided chloroplast clustering. Our results indicate that salt stress triggered the chloroplast clumping and affected the photosynthesis in O. streptacantha chlorenchyma cells. The exogenous catalase reverted the H2O2 accumulation and clustering of chloroplast, which led to an improvement of the photosynthetic efficiency. These data suggest that H2O2 detoxification by catalase is important to protect the chloroplast, thus conserving the photosynthetic activity in O. streptacantha under stress.
format	article
author	Diana Marcela Arias-Moreno Juan Francisco Jiménez-Bremont Israel Maruri-López Pablo Delgado-Sánchez
author_facet	Diana Marcela Arias-Moreno Juan Francisco Jiménez-Bremont Israel Maruri-López Pablo Delgado-Sánchez
author_sort	Diana Marcela Arias-Moreno
title	Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
title_short	Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
title_full	Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
title_fullStr	Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
title_full_unstemmed	Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress
title_sort	effects of catalase on chloroplast arrangement in opuntia streptacantha chlorenchyma cells under salt stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/70d3cf5aa95547119a072d8cca9a4957
work_keys_str_mv	AT dianamarcelaariasmoreno effectsofcatalaseonchloroplastarrangementinopuntiastreptacanthachlorenchymacellsundersaltstress AT juanfranciscojimenezbremont effectsofcatalaseonchloroplastarrangementinopuntiastreptacanthachlorenchymacellsundersaltstress AT israelmarurilopez effectsofcatalaseonchloroplastarrangementinopuntiastreptacanthachlorenchymacellsundersaltstress AT pablodelgadosanchez effectsofcatalaseonchloroplastarrangementinopuntiastreptacanthachlorenchymacellsundersaltstress
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Effects of catalase on chloroplast arrangement in Opuntia streptacantha chlorenchyma cells under salt stress

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