Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex

In some systems, a single protein comprising reverse transcriptase (RT), integrase and maturase enables concerted sequence integration and crRNA production. Here, analyses including the structure of a Cas6-RT-Cas1—Cas2 complex suggest coordination between all three active sites and capacity to acqui...

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Autores principales:	Joy Y. Wang, Christopher M. Hoel, Basem Al-Shayeb, Jillian F. Banfield, Stephen G. Brohawn, Jennifer A. Doudna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/70d3d3cf8bd24688beb348b37ccc83fb
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spelling	oai:doaj.org-article:70d3d3cf8bd24688beb348b37ccc83fb2021-12-02T14:49:31ZStructural coordination between active sites of a CRISPR reverse transcriptase-integrase complex10.1038/s41467-021-22900-y2041-1723https://doaj.org/article/70d3d3cf8bd24688beb348b37ccc83fb2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22900-yhttps://doaj.org/toc/2041-1723In some systems, a single protein comprising reverse transcriptase (RT), integrase and maturase enables concerted sequence integration and crRNA production. Here, analyses including the structure of a Cas6-RT-Cas1—Cas2 complex suggest coordination between all three active sites and capacity to acquire CRISPR sequences from RNA and DNA substrates.Joy Y. WangChristopher M. HoelBasem Al-ShayebJillian F. BanfieldStephen G. BrohawnJennifer A. DoudnaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joy Y. Wang Christopher M. Hoel Basem Al-Shayeb Jillian F. Banfield Stephen G. Brohawn Jennifer A. Doudna Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
description	In some systems, a single protein comprising reverse transcriptase (RT), integrase and maturase enables concerted sequence integration and crRNA production. Here, analyses including the structure of a Cas6-RT-Cas1—Cas2 complex suggest coordination between all three active sites and capacity to acquire CRISPR sequences from RNA and DNA substrates.
format	article
author	Joy Y. Wang Christopher M. Hoel Basem Al-Shayeb Jillian F. Banfield Stephen G. Brohawn Jennifer A. Doudna
author_facet	Joy Y. Wang Christopher M. Hoel Basem Al-Shayeb Jillian F. Banfield Stephen G. Brohawn Jennifer A. Doudna
author_sort	Joy Y. Wang
title	Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
title_short	Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
title_full	Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
title_fullStr	Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
title_full_unstemmed	Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex
title_sort	structural coordination between active sites of a crispr reverse transcriptase-integrase complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70d3d3cf8bd24688beb348b37ccc83fb
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Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex

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