Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Current HCV nucleic acid-based diagnosis is largely performed in centralised laboratories. Here, the authors present a pan-genotypic RNA assay, based on reverse transcriptase loop mediated isothermal amplification and develop a low-cost prototype paper-based lateral flow device for point-of-care use...

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Autores principales:	Weronika Witkowska McConnell, Chris Davis, Suleman R. Sabir, Alice Garrett, Amanda Bradley-Stewart, Pawel Jajesniak, Julien Reboud, Gaolian Xu, Zhugen Yang, Rory Gunson, Emma C. Thomson, Jonathan M. Cooper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/70d48ed159ba44c5ab33a8ce3a115976
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spelling	oai:doaj.org-article:70d48ed159ba44c5ab33a8ce3a1159762021-12-05T12:22:23ZPaper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus10.1038/s41467-021-27076-z2041-1723https://doaj.org/article/70d48ed159ba44c5ab33a8ce3a1159762021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27076-zhttps://doaj.org/toc/2041-1723Current HCV nucleic acid-based diagnosis is largely performed in centralised laboratories. Here, the authors present a pan-genotypic RNA assay, based on reverse transcriptase loop mediated isothermal amplification and develop a low-cost prototype paper-based lateral flow device for point-of-care use, providing a visually read result within 40 min.Weronika Witkowska McConnellChris DavisSuleman R. SabirAlice GarrettAmanda Bradley-StewartPawel JajesniakJulien ReboudGaolian XuZhugen YangRory GunsonEmma C. ThomsonJonathan M. CooperNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Weronika Witkowska McConnell Chris Davis Suleman R. Sabir Alice Garrett Amanda Bradley-Stewart Pawel Jajesniak Julien Reboud Gaolian Xu Zhugen Yang Rory Gunson Emma C. Thomson Jonathan M. Cooper Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
description	Current HCV nucleic acid-based diagnosis is largely performed in centralised laboratories. Here, the authors present a pan-genotypic RNA assay, based on reverse transcriptase loop mediated isothermal amplification and develop a low-cost prototype paper-based lateral flow device for point-of-care use, providing a visually read result within 40 min.
format	article
author	Weronika Witkowska McConnell Chris Davis Suleman R. Sabir Alice Garrett Amanda Bradley-Stewart Pawel Jajesniak Julien Reboud Gaolian Xu Zhugen Yang Rory Gunson Emma C. Thomson Jonathan M. Cooper
author_facet	Weronika Witkowska McConnell Chris Davis Suleman R. Sabir Alice Garrett Amanda Bradley-Stewart Pawel Jajesniak Julien Reboud Gaolian Xu Zhugen Yang Rory Gunson Emma C. Thomson Jonathan M. Cooper
author_sort	Weronika Witkowska McConnell
title	Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
title_short	Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
title_full	Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
title_fullStr	Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
title_full_unstemmed	Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus
title_sort	paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis c virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70d48ed159ba44c5ab33a8ce3a115976
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Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

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