Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol

Abstract To realize the economical production of ethanol and other bio-based chemicals from lignocellulosic biomass by consolidated bioprocessing (CBP), various cellulases from different sources were tested to improve the level of cellulase secretion in the yeast Saccharomyces cerevisiae by screenin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cho-Ryong Lee, Bong Hyun Sung, Kwang-Mook Lim, Mi-Jin Kim, Min Jeong Sohn, Jung-Hoon Bae, Jung-Hoon Sohn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70e49ef4841a4f39942fdfe47db5519c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70e49ef4841a4f39942fdfe47db5519c
record_format	dspace
spelling	oai:doaj.org-article:70e49ef4841a4f39942fdfe47db5519c2021-12-02T16:06:32ZCo-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol10.1038/s41598-017-04815-12045-2322https://doaj.org/article/70e49ef4841a4f39942fdfe47db5519c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04815-1https://doaj.org/toc/2045-2322Abstract To realize the economical production of ethanol and other bio-based chemicals from lignocellulosic biomass by consolidated bioprocessing (CBP), various cellulases from different sources were tested to improve the level of cellulase secretion in the yeast Saccharomyces cerevisiae by screening an optimal translational fusion partner (TFP) as both a secretion signal and fusion partner. Among them, four indispensable cellulases for cellulose hydrolysis, including Chaetomium thermophilum cellobiohydrolase (CtCBH1), Chrysosporium lucknowense cellobiohydrolase (ClCBH2), Trichoderma reesei endoglucanase (TrEGL2), and Saccharomycopsis fibuligera β-glucosidase (SfBGL1), were identified to be highly secreted in active form in yeast. Despite variability in the enzyme levels produced, each recombinant yeast could secrete approximately 0.6–2.0 g/L of cellulases into the fermentation broth. The synergistic effect of the mixed culture of the four strains expressing the essential cellulases with the insoluble substrate Avicel and several types of cellulosic biomass was demonstrated to be effective. Co-fermentation of these yeast strains produced approximately 14 g/L ethanol from the pre-treated rice straw containing 35 g/L glucan with 3-fold higher productivity than that of wild type yeast using a reduced amount of commercial cellulases. This process will contribute to the cost-effective production of bioenergy such as bioethanol and biochemicals from cellulosic biomass.Cho-Ryong LeeBong Hyun SungKwang-Mook LimMi-Jin KimMin Jeong SohnJung-Hoon BaeJung-Hoon SohnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cho-Ryong Lee Bong Hyun Sung Kwang-Mook Lim Mi-Jin Kim Min Jeong Sohn Jung-Hoon Bae Jung-Hoon Sohn Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
description	Abstract To realize the economical production of ethanol and other bio-based chemicals from lignocellulosic biomass by consolidated bioprocessing (CBP), various cellulases from different sources were tested to improve the level of cellulase secretion in the yeast Saccharomyces cerevisiae by screening an optimal translational fusion partner (TFP) as both a secretion signal and fusion partner. Among them, four indispensable cellulases for cellulose hydrolysis, including Chaetomium thermophilum cellobiohydrolase (CtCBH1), Chrysosporium lucknowense cellobiohydrolase (ClCBH2), Trichoderma reesei endoglucanase (TrEGL2), and Saccharomycopsis fibuligera β-glucosidase (SfBGL1), were identified to be highly secreted in active form in yeast. Despite variability in the enzyme levels produced, each recombinant yeast could secrete approximately 0.6–2.0 g/L of cellulases into the fermentation broth. The synergistic effect of the mixed culture of the four strains expressing the essential cellulases with the insoluble substrate Avicel and several types of cellulosic biomass was demonstrated to be effective. Co-fermentation of these yeast strains produced approximately 14 g/L ethanol from the pre-treated rice straw containing 35 g/L glucan with 3-fold higher productivity than that of wild type yeast using a reduced amount of commercial cellulases. This process will contribute to the cost-effective production of bioenergy such as bioethanol and biochemicals from cellulosic biomass.
format	article
author	Cho-Ryong Lee Bong Hyun Sung Kwang-Mook Lim Mi-Jin Kim Min Jeong Sohn Jung-Hoon Bae Jung-Hoon Sohn
author_facet	Cho-Ryong Lee Bong Hyun Sung Kwang-Mook Lim Mi-Jin Kim Min Jeong Sohn Jung-Hoon Bae Jung-Hoon Sohn
author_sort	Cho-Ryong Lee
title	Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
title_short	Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
title_full	Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
title_fullStr	Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
title_full_unstemmed	Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
title_sort	co-fermentation using recombinant saccharomyces cerevisiae yeast strains hyper-secreting different cellulases for the production of cellulosic bioethanol
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/70e49ef4841a4f39942fdfe47db5519c
work_keys_str_mv	AT choryonglee cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT bonghyunsung cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT kwangmooklim cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT mijinkim cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT minjeongsohn cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT junghoonbae cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol AT junghoonsohn cofermentationusingrecombinantsaccharomycescerevisiaeyeaststrainshypersecretingdifferentcellulasesfortheproductionofcellulosicbioethanol
_version_	1718384967732428800

Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol

Ejemplares similares