Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus.
Dietary composition largely influences pig's gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial co...
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2014
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oai:doaj.org-article:70eb3fcdb24f452b91ff9f71d2d5ced92021-11-18T08:36:00ZMucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus.1932-620310.1371/journal.pone.0086950https://doaj.org/article/70eb3fcdb24f452b91ff9f71d2d5ced92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466298/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dietary composition largely influences pig's gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. In the present study, we characterized the mucosa-associated bacterial communities at the Pars non-glandularis of the stomach, ileum and colon, and identified shifts in these communities in response to different dietary calcium-phosphorus (Ca-P) contents (100% versus 190% of the Ca and P requirements) in combination with two basal diets (wheat-barley- or corn-based) in weaned pigs. Pyrosequencing of 16S rRNA genes from 93 mucosal samples yielded 447,849 sequences, clustering into 997 operational taxonomic units (OTUs) at 97% similarity level. OTUs were assigned to 198 genera belonging to 14 different phyla. Correlation-based networks revealed strong interactions among OTUs at the various gastrointestinal sites. Our data describe a previously not reported high diversity and species richness at the Pars non-glandularis of the stomach in weaned pigs. Moreover, high versus adequate Ca-P content significantly promoted Lactobacillus by 14.9% units (1.4 fold change) at the gastric Pars non-glandularis (P = 0.035). Discriminant analysis revealed dynamic changes in OTU composition in response to dietary cereals and Ca-P contents at all gastrointestinal sites which were less distinguishable at higher taxonomic levels. Overall, this study revealed a distinct mucosa-associated bacterial community at the different gut sites, and a strong effect of high Ca-P diets on the gastric community, thereby markedly expanding our comprehension on mucosa-associated microbiota and their diet-related dynamics in weaned pigs.Evelyne MannStephan Schmitz-EsserQendrim ZebeliMartin WagnerMathias RitzmannBarbara U Metzler-ZebeliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86950 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Evelyne Mann Stephan Schmitz-Esser Qendrim Zebeli Martin Wagner Mathias Ritzmann Barbara U Metzler-Zebeli Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| description |
Dietary composition largely influences pig's gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. In the present study, we characterized the mucosa-associated bacterial communities at the Pars non-glandularis of the stomach, ileum and colon, and identified shifts in these communities in response to different dietary calcium-phosphorus (Ca-P) contents (100% versus 190% of the Ca and P requirements) in combination with two basal diets (wheat-barley- or corn-based) in weaned pigs. Pyrosequencing of 16S rRNA genes from 93 mucosal samples yielded 447,849 sequences, clustering into 997 operational taxonomic units (OTUs) at 97% similarity level. OTUs were assigned to 198 genera belonging to 14 different phyla. Correlation-based networks revealed strong interactions among OTUs at the various gastrointestinal sites. Our data describe a previously not reported high diversity and species richness at the Pars non-glandularis of the stomach in weaned pigs. Moreover, high versus adequate Ca-P content significantly promoted Lactobacillus by 14.9% units (1.4 fold change) at the gastric Pars non-glandularis (P = 0.035). Discriminant analysis revealed dynamic changes in OTU composition in response to dietary cereals and Ca-P contents at all gastrointestinal sites which were less distinguishable at higher taxonomic levels. Overall, this study revealed a distinct mucosa-associated bacterial community at the different gut sites, and a strong effect of high Ca-P diets on the gastric community, thereby markedly expanding our comprehension on mucosa-associated microbiota and their diet-related dynamics in weaned pigs. |
| format |
article |
| author |
Evelyne Mann Stephan Schmitz-Esser Qendrim Zebeli Martin Wagner Mathias Ritzmann Barbara U Metzler-Zebeli |
| author_facet |
Evelyne Mann Stephan Schmitz-Esser Qendrim Zebeli Martin Wagner Mathias Ritzmann Barbara U Metzler-Zebeli |
| author_sort |
Evelyne Mann |
| title |
Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| title_short |
Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| title_full |
Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| title_fullStr |
Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| title_full_unstemmed |
Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| title_sort |
mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/70eb3fcdb24f452b91ff9f71d2d5ced9 |
| work_keys_str_mv |
AT evelynemann mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus AT stephanschmitzesser mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus AT qendrimzebeli mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus AT martinwagner mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus AT mathiasritzmann mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus AT barbaraumetzlerzebeli mucosaassociatedbacterialmicrobiomeofthegastrointestinaltractofweanedpigsanddynamicslinkedtodietarycalciumphosphorus |
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