Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography

Abstract We developed a method to measure the relative blood flow speed using optical coherence tomography angiography (OCTA) in retina and choroid, and investigated the feasibility of this method for assessing microcirculatory function in rat models of sepsis and hemorrhagic shock. Two sepsis model...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jang Ryul Park, ByungKun Lee, Min Ji Lee, Kyuseok Kim, Wang-Yuhl Oh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70ef14ba53d94778b9449a832cb43c5a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70ef14ba53d94778b9449a832cb43c5a
record_format	dspace
spelling	oai:doaj.org-article:70ef14ba53d94778b9449a832cb43c5a2021-12-02T16:14:09ZVisualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography10.1038/s41598-021-93631-92045-2322https://doaj.org/article/70ef14ba53d94778b9449a832cb43c5a2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93631-9https://doaj.org/toc/2045-2322Abstract We developed a method to measure the relative blood flow speed using optical coherence tomography angiography (OCTA) in retina and choroid, and investigated the feasibility of this method for assessing microcirculatory function in rat models of sepsis and hemorrhagic shock. Two sepsis models, 6-h severe sepsis without treatment and 30-h moderate sepsis maintaining mean arterial pressure, and volume controlled hemorrhagic shock and fluid resuscitation model were used to see the change of microcirculation. The blood flow index (BFI), which was calculated from the OCTA images to represent the average relative blood flow, was decreasing during the 6-h severe sepsis model. Its change is in parallel with the mean arterial blood pressure (MAP) and blood lactate levels. In the 30-h moderate sepsis model, the BFI was decreased while maintaining MAP, and lactate was increased. In the hemorrhagic shock model, the change of BFI is in line with MAP and lactate levels. In all models, BFI change is more sensitive in choroid than in retina. This study presents the OCTA-based retinal and choroidal microcirculatory blood flow monitoring method and shows its utility for assessment of critical illness.Jang Ryul ParkByungKun LeeMin Ji LeeKyuseok KimWang-Yuhl OhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jang Ryul Park ByungKun Lee Min Ji Lee Kyuseok Kim Wang-Yuhl Oh Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
description	Abstract We developed a method to measure the relative blood flow speed using optical coherence tomography angiography (OCTA) in retina and choroid, and investigated the feasibility of this method for assessing microcirculatory function in rat models of sepsis and hemorrhagic shock. Two sepsis models, 6-h severe sepsis without treatment and 30-h moderate sepsis maintaining mean arterial pressure, and volume controlled hemorrhagic shock and fluid resuscitation model were used to see the change of microcirculation. The blood flow index (BFI), which was calculated from the OCTA images to represent the average relative blood flow, was decreasing during the 6-h severe sepsis model. Its change is in parallel with the mean arterial blood pressure (MAP) and blood lactate levels. In the 30-h moderate sepsis model, the BFI was decreased while maintaining MAP, and lactate was increased. In the hemorrhagic shock model, the change of BFI is in line with MAP and lactate levels. In all models, BFI change is more sensitive in choroid than in retina. This study presents the OCTA-based retinal and choroidal microcirculatory blood flow monitoring method and shows its utility for assessment of critical illness.
format	article
author	Jang Ryul Park ByungKun Lee Min Ji Lee Kyuseok Kim Wang-Yuhl Oh
author_facet	Jang Ryul Park ByungKun Lee Min Ji Lee Kyuseok Kim Wang-Yuhl Oh
author_sort	Jang Ryul Park
title	Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
title_short	Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
title_full	Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
title_fullStr	Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
title_full_unstemmed	Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
title_sort	visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70ef14ba53d94778b9449a832cb43c5a
work_keys_str_mv	AT jangryulpark visualizationofthreedimensionalmicrocirculationofrodentsretinaandchoroidforstudiesofcriticalillnessusingopticalcoherencetomographyangiography AT byungkunlee visualizationofthreedimensionalmicrocirculationofrodentsretinaandchoroidforstudiesofcriticalillnessusingopticalcoherencetomographyangiography AT minjilee visualizationofthreedimensionalmicrocirculationofrodentsretinaandchoroidforstudiesofcriticalillnessusingopticalcoherencetomographyangiography AT kyuseokkim visualizationofthreedimensionalmicrocirculationofrodentsretinaandchoroidforstudiesofcriticalillnessusingopticalcoherencetomographyangiography AT wangyuhloh visualizationofthreedimensionalmicrocirculationofrodentsretinaandchoroidforstudiesofcriticalillnessusingopticalcoherencetomographyangiography
_version_	1718384356481826816

Visualization of three-dimensional microcirculation of rodents’ retina and choroid for studies of critical illness using optical coherence tomography angiography

Ejemplares similares