In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions

Within the adult canine population, disabilities and symptoms including joint pain and functional impairment are commonly observed in articular cartilage lesions and present a challenging feat in the operating room. Clinical settings require less invasive and more minimally manipulated measures faci...

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Autores principales:	Francesco De Francesco, Valentina Riccio, Alice Busato, Caterina Di Bella, Evelina Serri, Andrea Sbarbati, Barbara Zavan, Michele Riccio, Angela Palumbo Piccionello
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microfragmented adipose tissue micrografts Rigenera technology adipose stem cells canine adipose tissue Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/70f18d351c814ac59e31d6485b1b945b
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spelling	oai:doaj.org-article:70f18d351c814ac59e31d6485b1b945b2021-11-25T16:19:36ZIn Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions10.3390/ani111132312076-2615https://doaj.org/article/70f18d351c814ac59e31d6485b1b945b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3231https://doaj.org/toc/2076-2615Within the adult canine population, disabilities and symptoms including joint pain and functional impairment are commonly observed in articular cartilage lesions and present a challenging feat in the operating room. Clinical settings require less invasive and more minimally manipulated measures facilitated by innovative and advanced technology. Mesenchymal stem cells have recently been proposed and, furthermore, autologous adipose tissue administration via injection has emerged as a new albeit somewhat controversial therapeutic tool. The purpose of this study is to characterize canine autologous micro-fragmented adipose tissue (micrografts) by mechanical approach without substantial manipulations. Adipose tissue samples collected from six dogs were processed by a Rigenera device and by enzymatic digestion from two different body regions (lumbar and thigh region). Interestingly, the immunophenotypic analysis attested that cells from Rigenera<sup>®</sup> were highly positive for the mesenchymal stem cells markers CD73 and CD90, less positive for hematopoietic CD45 and CD34, and negative for MHC class II antibodies (which play a role in immune responses). Finally, the Rigenera<sup>®</sup> technology obtained micrografts with a 35% higher expression of the IL10 gene with relevant anti-inflammatory activities compared to the enzymatic digestion protocol. This evidence suggests a potential improved clinical outcome capable of modulating inflammation and immune responses.Francesco De FrancescoValentina RiccioAlice BusatoCaterina Di BellaEvelina SerriAndrea SbarbatiBarbara ZavanMichele RiccioAngela Palumbo PiccionelloMDPI AGarticlemicrofragmented adipose tissuemicrograftsRigenera technologyadipose stem cellscanine adipose tissueVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3231, p 3231 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfragmented adipose tissue micrografts Rigenera technology adipose stem cells canine adipose tissue Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	microfragmented adipose tissue micrografts Rigenera technology adipose stem cells canine adipose tissue Veterinary medicine SF600-1100 Zoology QL1-991 Francesco De Francesco Valentina Riccio Alice Busato Caterina Di Bella Evelina Serri Andrea Sbarbati Barbara Zavan Michele Riccio Angela Palumbo Piccionello In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
description	Within the adult canine population, disabilities and symptoms including joint pain and functional impairment are commonly observed in articular cartilage lesions and present a challenging feat in the operating room. Clinical settings require less invasive and more minimally manipulated measures facilitated by innovative and advanced technology. Mesenchymal stem cells have recently been proposed and, furthermore, autologous adipose tissue administration via injection has emerged as a new albeit somewhat controversial therapeutic tool. The purpose of this study is to characterize canine autologous micro-fragmented adipose tissue (micrografts) by mechanical approach without substantial manipulations. Adipose tissue samples collected from six dogs were processed by a Rigenera device and by enzymatic digestion from two different body regions (lumbar and thigh region). Interestingly, the immunophenotypic analysis attested that cells from Rigenera<sup>®</sup> were highly positive for the mesenchymal stem cells markers CD73 and CD90, less positive for hematopoietic CD45 and CD34, and negative for MHC class II antibodies (which play a role in immune responses). Finally, the Rigenera<sup>®</sup> technology obtained micrografts with a 35% higher expression of the IL10 gene with relevant anti-inflammatory activities compared to the enzymatic digestion protocol. This evidence suggests a potential improved clinical outcome capable of modulating inflammation and immune responses.
format	article
author	Francesco De Francesco Valentina Riccio Alice Busato Caterina Di Bella Evelina Serri Andrea Sbarbati Barbara Zavan Michele Riccio Angela Palumbo Piccionello
author_facet	Francesco De Francesco Valentina Riccio Alice Busato Caterina Di Bella Evelina Serri Andrea Sbarbati Barbara Zavan Michele Riccio Angela Palumbo Piccionello
author_sort	Francesco De Francesco
title	In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
title_short	In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
title_full	In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
title_fullStr	In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
title_full_unstemmed	In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions
title_sort	in vitro characterization of canine microfragmented adipose tissue non-enzymatically extracted from the thigh and lumbar regions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/70f18d351c814ac59e31d6485b1b945b
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In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions

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