Hypersaline sapropels act as hotspots for microbial dark matter

Abstract Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habit...

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Autores principales:	Adrian-Ştefan Andrei, Andreea Baricz, Michael Scott Robeson, Manuela Raluca Păuşan, Tudor Tămaş, Cecilia Chiriac, Edina Szekeres, Lucian Barbu-Tudoran, Erika Andrea Levei, Cristian Coman, Mircea Podar, Horia Leonard Banciu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70f2dc1bed9e4b3aabae201bd3e2446b
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spelling	oai:doaj.org-article:70f2dc1bed9e4b3aabae201bd3e2446b2021-12-02T12:32:00ZHypersaline sapropels act as hotspots for microbial dark matter10.1038/s41598-017-06232-w2045-2322https://doaj.org/article/70f2dc1bed9e4b3aabae201bd3e2446b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06232-whttps://doaj.org/toc/2045-2322Abstract Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.Adrian-Ştefan AndreiAndreea BariczMichael Scott RobesonManuela Raluca PăuşanTudor TămaşCecilia ChiriacEdina SzekeresLucian Barbu-TudoranErika Andrea LeveiCristian ComanMircea PodarHoria Leonard BanciuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrian-Ştefan Andrei Andreea Baricz Michael Scott Robeson Manuela Raluca Păuşan Tudor Tămaş Cecilia Chiriac Edina Szekeres Lucian Barbu-Tudoran Erika Andrea Levei Cristian Coman Mircea Podar Horia Leonard Banciu Hypersaline sapropels act as hotspots for microbial dark matter
description	Abstract Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.
format	article
author	Adrian-Ştefan Andrei Andreea Baricz Michael Scott Robeson Manuela Raluca Păuşan Tudor Tămaş Cecilia Chiriac Edina Szekeres Lucian Barbu-Tudoran Erika Andrea Levei Cristian Coman Mircea Podar Horia Leonard Banciu
author_facet	Adrian-Ştefan Andrei Andreea Baricz Michael Scott Robeson Manuela Raluca Păuşan Tudor Tămaş Cecilia Chiriac Edina Szekeres Lucian Barbu-Tudoran Erika Andrea Levei Cristian Coman Mircea Podar Horia Leonard Banciu
author_sort	Adrian-Ştefan Andrei
title	Hypersaline sapropels act as hotspots for microbial dark matter
title_short	Hypersaline sapropels act as hotspots for microbial dark matter
title_full	Hypersaline sapropels act as hotspots for microbial dark matter
title_fullStr	Hypersaline sapropels act as hotspots for microbial dark matter
title_full_unstemmed	Hypersaline sapropels act as hotspots for microbial dark matter
title_sort	hypersaline sapropels act as hotspots for microbial dark matter
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/70f2dc1bed9e4b3aabae201bd3e2446b
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Hypersaline sapropels act as hotspots for microbial dark matter

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