Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198

Abstract Background Circular RNAs (circRNAs) are pivotal regulators of various human cancers and circ-ERBB2 is abnormally expressed in breast cancer cells. However, the role and mechanism of circ-ERBB2 in HER2-positive breast cancer are still unknown. Methods The circ-ERBB2 expressions in the tumor...

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Autores principales:	Jin-xiu Zhong, Yun-yuan Kong, Rong-guang Luo, Guo-jin Xia, Wen-xing He, Xue-zhong Chen, Wei-wei Tan, Qing-jie Chen, Yu-yin Huang, Yan-xing Guan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Circ-ERBB2 miR-136-5p miR-198 TFAP2C HER2-positive breast cancer Medicine R
Acceso en línea:	https://doaj.org/article/70f539635ee245c0bf9a15d32195a0dd
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spelling	oai:doaj.org-article:70f539635ee245c0bf9a15d32195a0dd2021-11-07T12:08:22ZCircular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-19810.1186/s12967-021-03114-81479-5876https://doaj.org/article/70f539635ee245c0bf9a15d32195a0dd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12967-021-03114-8https://doaj.org/toc/1479-5876Abstract Background Circular RNAs (circRNAs) are pivotal regulators of various human cancers and circ-ERBB2 is abnormally expressed in breast cancer cells. However, the role and mechanism of circ-ERBB2 in HER2-positive breast cancer are still unknown. Methods The circ-ERBB2 expressions in the tumor tissues of HER2-positive breast cancer patients were tested using quantitative real-time PCR. The circ-ERBB2 function was investigated by cell counting kit 8 assay, Transwell, flow cytometry and Western blot. Mechanistically, fluorescence in situ hybridization, RNA immunoprecipitation, RNA pull-down and dual-luciferase reporter gene assays were conducted to confirm the interaction between circ-ERBB2 and miR-136-5p or miR-198 in HER2-positive breast cancer cells. Results Circ-ERBB2 was elevated in the tumor tissues of HER2-positive breast cancer patients. Functionally, the interference with circ-ERBB2 repressed HER2-positive breast cancer cell proliferation, migration, invasion and accelerated cell apoptosis. Furthermore, the mechanistic analysis corroborated that circ-ERBB2 acted as a competing endogenous RNA for miR-136-5p or miR-198 to relieve the repressive influence of miR-136-5p or miR-198 on its target transcription factor activator protein 2C (TFAP2C). Meanwhile, in vivo assays further corroborated the oncogenic function of circ-ERBB2 in HER2-positive breast cancer. Conclusions Circ-ERBB2 accelerated HER2-positive breast cancer progression through the circ-ERBB2/miR-136-5p/TFAP2C axis or the circ-ERBB2/miR-198/TFAP2C axis.Jin-xiu ZhongYun-yuan KongRong-guang LuoGuo-jin XiaWen-xing HeXue-zhong ChenWei-wei TanQing-jie ChenYu-yin HuangYan-xing GuanBMCarticleCirc-ERBB2miR-136-5pmiR-198TFAP2CHER2-positive breast cancerMedicineRENJournal of Translational Medicine, Vol 19, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Circ-ERBB2 miR-136-5p miR-198 TFAP2C HER2-positive breast cancer Medicine R
spellingShingle	Circ-ERBB2 miR-136-5p miR-198 TFAP2C HER2-positive breast cancer Medicine R Jin-xiu Zhong Yun-yuan Kong Rong-guang Luo Guo-jin Xia Wen-xing He Xue-zhong Chen Wei-wei Tan Qing-jie Chen Yu-yin Huang Yan-xing Guan Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
description	Abstract Background Circular RNAs (circRNAs) are pivotal regulators of various human cancers and circ-ERBB2 is abnormally expressed in breast cancer cells. However, the role and mechanism of circ-ERBB2 in HER2-positive breast cancer are still unknown. Methods The circ-ERBB2 expressions in the tumor tissues of HER2-positive breast cancer patients were tested using quantitative real-time PCR. The circ-ERBB2 function was investigated by cell counting kit 8 assay, Transwell, flow cytometry and Western blot. Mechanistically, fluorescence in situ hybridization, RNA immunoprecipitation, RNA pull-down and dual-luciferase reporter gene assays were conducted to confirm the interaction between circ-ERBB2 and miR-136-5p or miR-198 in HER2-positive breast cancer cells. Results Circ-ERBB2 was elevated in the tumor tissues of HER2-positive breast cancer patients. Functionally, the interference with circ-ERBB2 repressed HER2-positive breast cancer cell proliferation, migration, invasion and accelerated cell apoptosis. Furthermore, the mechanistic analysis corroborated that circ-ERBB2 acted as a competing endogenous RNA for miR-136-5p or miR-198 to relieve the repressive influence of miR-136-5p or miR-198 on its target transcription factor activator protein 2C (TFAP2C). Meanwhile, in vivo assays further corroborated the oncogenic function of circ-ERBB2 in HER2-positive breast cancer. Conclusions Circ-ERBB2 accelerated HER2-positive breast cancer progression through the circ-ERBB2/miR-136-5p/TFAP2C axis or the circ-ERBB2/miR-198/TFAP2C axis.
format	article
author	Jin-xiu Zhong Yun-yuan Kong Rong-guang Luo Guo-jin Xia Wen-xing He Xue-zhong Chen Wei-wei Tan Qing-jie Chen Yu-yin Huang Yan-xing Guan
author_facet	Jin-xiu Zhong Yun-yuan Kong Rong-guang Luo Guo-jin Xia Wen-xing He Xue-zhong Chen Wei-wei Tan Qing-jie Chen Yu-yin Huang Yan-xing Guan
author_sort	Jin-xiu Zhong
title	Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
title_short	Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
title_full	Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
title_fullStr	Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
title_full_unstemmed	Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198
title_sort	circular rna circ-erbb2 promotes her2-positive breast cancer progression and metastasis via sponging mir-136-5p and mir-198
publisher	BMC
publishDate	2021
url	https://doaj.org/article/70f539635ee245c0bf9a15d32195a0dd
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Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198

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