Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases

Ye et al demonstrate a simple and precise method to simultaneously generate iPSC lines with different gene dosages using paired Cas9 nickases. As proof-of-concept they apply this method to examining amyloid precursor protein gene dosage effects in an Alzheimer’s disease patient-derived iPSC line. Th...

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Autores principales:	Tao Ye, Yangyang Duan, Hayley W. S. Tsang, He Xu, Yuewen Chen, Han Cao, Yu Chen, Amy K. Y. Fu, Nancy Y. Ip
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/70fb7c21e42e47428a1ea40e2a8b140e
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spelling	oai:doaj.org-article:70fb7c21e42e47428a1ea40e2a8b140e2021-12-02T13:30:08ZEfficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases10.1038/s42003-021-01722-02399-3642https://doaj.org/article/70fb7c21e42e47428a1ea40e2a8b140e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01722-0https://doaj.org/toc/2399-3642Ye et al demonstrate a simple and precise method to simultaneously generate iPSC lines with different gene dosages using paired Cas9 nickases. As proof-of-concept they apply this method to examining amyloid precursor protein gene dosage effects in an Alzheimer’s disease patient-derived iPSC line. Their method could potentially advance what we know about disease mechanisms and assist with gene therapy development.Tao YeYangyang DuanHayley W. S. TsangHe XuYuewen ChenHan CaoYu ChenAmy K. Y. FuNancy Y. IpNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Tao Ye Yangyang Duan Hayley W. S. Tsang He Xu Yuewen Chen Han Cao Yu Chen Amy K. Y. Fu Nancy Y. Ip Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
description	Ye et al demonstrate a simple and precise method to simultaneously generate iPSC lines with different gene dosages using paired Cas9 nickases. As proof-of-concept they apply this method to examining amyloid precursor protein gene dosage effects in an Alzheimer’s disease patient-derived iPSC line. Their method could potentially advance what we know about disease mechanisms and assist with gene therapy development.
format	article
author	Tao Ye Yangyang Duan Hayley W. S. Tsang He Xu Yuewen Chen Han Cao Yu Chen Amy K. Y. Fu Nancy Y. Ip
author_facet	Tao Ye Yangyang Duan Hayley W. S. Tsang He Xu Yuewen Chen Han Cao Yu Chen Amy K. Y. Fu Nancy Y. Ip
author_sort	Tao Ye
title	Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
title_short	Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
title_full	Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
title_fullStr	Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
title_full_unstemmed	Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases
title_sort	efficient manipulation of gene dosage in human ipscs using crispr/cas9 nickases
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/70fb7c21e42e47428a1ea40e2a8b140e
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Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases

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