Effects of Cations on the Behaviour of Lipid Cubic Phases

Abstract Inverse bicontinuous cubic structures formed by lipids have been demonstrated in a wide variety of applications, from a host matrix for proteins for crystallisation, to templates for nanoscale structures. Recent work has focused on tuning their properties to realize such applications, often...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christopher Brasnett, Georgia Longstaff, Laura Compton, Annela Seddon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70fdaa50791244778c6135d6ff37a6fa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70fdaa50791244778c6135d6ff37a6fa
record_format	dspace
spelling	oai:doaj.org-article:70fdaa50791244778c6135d6ff37a6fa2021-12-02T11:41:19ZEffects of Cations on the Behaviour of Lipid Cubic Phases10.1038/s41598-017-08438-42045-2322https://doaj.org/article/70fdaa50791244778c6135d6ff37a6fa2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08438-4https://doaj.org/toc/2045-2322Abstract Inverse bicontinuous cubic structures formed by lipids have been demonstrated in a wide variety of applications, from a host matrix for proteins for crystallisation, to templates for nanoscale structures. Recent work has focused on tuning their properties to realize such applications, often by manipulating the structure by introducing other lipids with different properties such as charge or packing. However, they are often prepared in the presence of solutions containing salt, counteracting the effects, for example, charged lipids, and fundamentally changing the structures obtained. Here, we demonstrate the delicate interplay between electrostatic swelling in bicontinuous structures formed by monoolein (MO) doped with both negatively charged dioleyl phosphatidylglycerol (DOPG), and zwitterionic dioleyl phosphatidylethanolamine (DOPE), with the addition of mono- and divalent salts. The effect of adding salt to the charged phase changes the structure from the primitive cubic ( $${{\bf{Q}}}_{II}^{P}$$ Q I I P ) to the double diamond phase ( $${{\bf{Q}}}_{II}^{D}$$ Q I I D ) whilst still allowing for modest increases in lattice parameter of up to a nanometer. Contrasting this, the addition of salts to the non-charged phase, has minimal effect on the lattice parameter but now the transition from the ( $${{\bf{Q}}}_{II}^{D}$$ Q I I D ) to the inverse hexagonal phase (H II ) is observed occurring at higher mole fractions of DOPE than in pure water.Christopher BrasnettGeorgia LongstaffLaura ComptonAnnela SeddonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher Brasnett Georgia Longstaff Laura Compton Annela Seddon Effects of Cations on the Behaviour of Lipid Cubic Phases
description	Abstract Inverse bicontinuous cubic structures formed by lipids have been demonstrated in a wide variety of applications, from a host matrix for proteins for crystallisation, to templates for nanoscale structures. Recent work has focused on tuning their properties to realize such applications, often by manipulating the structure by introducing other lipids with different properties such as charge or packing. However, they are often prepared in the presence of solutions containing salt, counteracting the effects, for example, charged lipids, and fundamentally changing the structures obtained. Here, we demonstrate the delicate interplay between electrostatic swelling in bicontinuous structures formed by monoolein (MO) doped with both negatively charged dioleyl phosphatidylglycerol (DOPG), and zwitterionic dioleyl phosphatidylethanolamine (DOPE), with the addition of mono- and divalent salts. The effect of adding salt to the charged phase changes the structure from the primitive cubic ( $${{\bf{Q}}}_{II}^{P}$$ Q I I P ) to the double diamond phase ( $${{\bf{Q}}}_{II}^{D}$$ Q I I D ) whilst still allowing for modest increases in lattice parameter of up to a nanometer. Contrasting this, the addition of salts to the non-charged phase, has minimal effect on the lattice parameter but now the transition from the ( $${{\bf{Q}}}_{II}^{D}$$ Q I I D ) to the inverse hexagonal phase (H II ) is observed occurring at higher mole fractions of DOPE than in pure water.
format	article
author	Christopher Brasnett Georgia Longstaff Laura Compton Annela Seddon
author_facet	Christopher Brasnett Georgia Longstaff Laura Compton Annela Seddon
author_sort	Christopher Brasnett
title	Effects of Cations on the Behaviour of Lipid Cubic Phases
title_short	Effects of Cations on the Behaviour of Lipid Cubic Phases
title_full	Effects of Cations on the Behaviour of Lipid Cubic Phases
title_fullStr	Effects of Cations on the Behaviour of Lipid Cubic Phases
title_full_unstemmed	Effects of Cations on the Behaviour of Lipid Cubic Phases
title_sort	effects of cations on the behaviour of lipid cubic phases
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/70fdaa50791244778c6135d6ff37a6fa
work_keys_str_mv	AT christopherbrasnett effectsofcationsonthebehaviouroflipidcubicphases AT georgialongstaff effectsofcationsonthebehaviouroflipidcubicphases AT lauracompton effectsofcationsonthebehaviouroflipidcubicphases AT annelaseddon effectsofcationsonthebehaviouroflipidcubicphases
_version_	1718395404623544320

Effects of Cations on the Behaviour of Lipid Cubic Phases

Ejemplares similares