C-Reactive Protein Controls IL-23 Production by Human Monocytes

C-reactive protein (CRP) is an acute-phase protein in humans that is produced in high quantities by the liver upon infection and under inflammatory conditions. Although CRP is commonly used as a marker of inflammation, CRP can also directly contribute to inflammation by eliciting pro-inflammatory cy...

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Autores principales:	Chiara E. Geyer, Melissa Newling, Lathees Sritharan, Guillermo R. Griffith, Hung-Jen Chen, Dominique L. P. Baeten, Jeroen den Dunnen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	C-reactive protein IL-23 inflammation monocyte Fc receptor Sepsis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7100eea814c24301a1c161057fb9f7a5
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id	oai:doaj.org-article:7100eea814c24301a1c161057fb9f7a5
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spelling	oai:doaj.org-article:7100eea814c24301a1c161057fb9f7a52021-11-11T17:06:48ZC-Reactive Protein Controls IL-23 Production by Human Monocytes10.3390/ijms2221116381422-00671661-6596https://doaj.org/article/7100eea814c24301a1c161057fb9f7a52021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11638https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067C-reactive protein (CRP) is an acute-phase protein in humans that is produced in high quantities by the liver upon infection and under inflammatory conditions. Although CRP is commonly used as a marker of inflammation, CRP can also directly contribute to inflammation by eliciting pro-inflammatory cytokine production by immune cells. Since CRP is highly elevated in serum under inflammatory conditions, we have studied the CRP-induced cytokine profile of human monocytes, one of the main innate immune cell populations in blood. We identified that CRP is relatively unique in its capacity to induce production of the pro-inflammatory cytokine IL-23, which was in stark contrast to a wide panel of pattern recognition receptor (PRR) ligands. We show that CRP-induced IL-23 production was mediated at the level of gene transcription, since CRP particularly promoted gene transcription of <i>IL23A</i> (encoding IL-23p19) instead of <i>IL12A</i> (encoding IL-12p35), while PRR ligands induce the opposite response. Interestingly, when CRP stimulation was combined with PRR ligand stimulation, as for example, occurs in the context of sepsis, IL-23 production by monocytes was strongly reduced. Combined, these data identify CRP as a unique individual ligand to induce IL-23 production by monocytes, which may contribute to shaping systemic immune responses under inflammatory conditions.Chiara E. GeyerMelissa NewlingLathees SritharanGuillermo R. GriffithHung-Jen ChenDominique L. P. BaetenJeroen den DunnenMDPI AGarticleC-reactive proteinIL-23inflammationmonocyteFc receptorSepsisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11638, p 11638 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	C-reactive protein IL-23 inflammation monocyte Fc receptor Sepsis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	C-reactive protein IL-23 inflammation monocyte Fc receptor Sepsis Biology (General) QH301-705.5 Chemistry QD1-999 Chiara E. Geyer Melissa Newling Lathees Sritharan Guillermo R. Griffith Hung-Jen Chen Dominique L. P. Baeten Jeroen den Dunnen C-Reactive Protein Controls IL-23 Production by Human Monocytes
description	C-reactive protein (CRP) is an acute-phase protein in humans that is produced in high quantities by the liver upon infection and under inflammatory conditions. Although CRP is commonly used as a marker of inflammation, CRP can also directly contribute to inflammation by eliciting pro-inflammatory cytokine production by immune cells. Since CRP is highly elevated in serum under inflammatory conditions, we have studied the CRP-induced cytokine profile of human monocytes, one of the main innate immune cell populations in blood. We identified that CRP is relatively unique in its capacity to induce production of the pro-inflammatory cytokine IL-23, which was in stark contrast to a wide panel of pattern recognition receptor (PRR) ligands. We show that CRP-induced IL-23 production was mediated at the level of gene transcription, since CRP particularly promoted gene transcription of <i>IL23A</i> (encoding IL-23p19) instead of <i>IL12A</i> (encoding IL-12p35), while PRR ligands induce the opposite response. Interestingly, when CRP stimulation was combined with PRR ligand stimulation, as for example, occurs in the context of sepsis, IL-23 production by monocytes was strongly reduced. Combined, these data identify CRP as a unique individual ligand to induce IL-23 production by monocytes, which may contribute to shaping systemic immune responses under inflammatory conditions.
format	article
author	Chiara E. Geyer Melissa Newling Lathees Sritharan Guillermo R. Griffith Hung-Jen Chen Dominique L. P. Baeten Jeroen den Dunnen
author_facet	Chiara E. Geyer Melissa Newling Lathees Sritharan Guillermo R. Griffith Hung-Jen Chen Dominique L. P. Baeten Jeroen den Dunnen
author_sort	Chiara E. Geyer
title	C-Reactive Protein Controls IL-23 Production by Human Monocytes
title_short	C-Reactive Protein Controls IL-23 Production by Human Monocytes
title_full	C-Reactive Protein Controls IL-23 Production by Human Monocytes
title_fullStr	C-Reactive Protein Controls IL-23 Production by Human Monocytes
title_full_unstemmed	C-Reactive Protein Controls IL-23 Production by Human Monocytes
title_sort	c-reactive protein controls il-23 production by human monocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7100eea814c24301a1c161057fb9f7a5
work_keys_str_mv	AT chiaraegeyer creactiveproteincontrolsil23productionbyhumanmonocytes AT melissanewling creactiveproteincontrolsil23productionbyhumanmonocytes AT latheessritharan creactiveproteincontrolsil23productionbyhumanmonocytes AT guillermorgriffith creactiveproteincontrolsil23productionbyhumanmonocytes AT hungjenchen creactiveproteincontrolsil23productionbyhumanmonocytes AT dominiquelpbaeten creactiveproteincontrolsil23productionbyhumanmonocytes AT jeroendendunnen creactiveproteincontrolsil23productionbyhumanmonocytes
_version_	1718432183622828032

C-Reactive Protein Controls IL-23 Production by Human Monocytes

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