From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species

Abstract Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study...

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Autores principales:	J. Ceusters, J.-Ph. Lejeune, C. Sandersen, A. Niesten, L. Lagneaux, D. Serteyn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7103b706ce834ef099d6579225801651
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spelling	oai:doaj.org-article:7103b706ce834ef099d65792258016512021-12-02T15:05:59ZFrom skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species10.1038/s41598-017-00803-72045-2322https://doaj.org/article/7103b706ce834ef099d65792258016512017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00803-7https://doaj.org/toc/2045-2322Abstract Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.J. CeustersJ.-Ph. LejeuneC. SandersenA. NiestenL. LagneauxD. SerteynNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. Ceusters J.-Ph. Lejeune C. Sandersen A. Niesten L. Lagneaux D. Serteyn From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
description	Abstract Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
format	article
author	J. Ceusters J.-Ph. Lejeune C. Sandersen A. Niesten L. Lagneaux D. Serteyn
author_facet	J. Ceusters J.-Ph. Lejeune C. Sandersen A. Niesten L. Lagneaux D. Serteyn
author_sort	J. Ceusters
title	From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
title_short	From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
title_full	From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
title_fullStr	From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
title_full_unstemmed	From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
title_sort	from skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7103b706ce834ef099d6579225801651
work_keys_str_mv	AT jceusters fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies AT jphlejeune fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies AT csandersen fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies AT aniesten fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies AT llagneaux fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies AT dserteyn fromskeletalmuscletostemcellsaninnovativeandminimallyinvasiveprocessformultiplespecies
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From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species

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