Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation

Abstract Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations resp...

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Autores principales:	Bo Eng Cheong, Olga Beine-Golovchuk, Michal Gorka, William Wing Ho Ho, Federico Martinez-Seidel, Alexandre Augusto Pereira Firmino, Aleksandra Skirycz, Ute Roessner, Joachim Kopka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/710717f23f624354a1201ec1e29d2780
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spelling	oai:doaj.org-article:710717f23f624354a1201ec1e29d27802021-12-02T14:16:58ZArabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation10.1038/s41598-021-81610-z2045-2322https://doaj.org/article/710717f23f624354a1201ec1e29d27802021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81610-zhttps://doaj.org/toc/2045-2322Abstract Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hyper-accumulation of non-translating subunits at steady-state temperature; after cold-shift, a KCl-sensitive 80S sub-fraction remains depleted. We infer that Arabidopsis may buffer fluctuating translation by pre-existing non-translating ribosomes before de novo synthesis meets temperature-induced demands. Reil1 reil2 double mutants accumulate 43S-preinitiation and pre-60S-maturation complexes and alter paralog composition of ribosomal proteins in non-translating complexes. With few exceptions, e.g. RPL3B and RPL24C, these changes are not under transcriptional control. Our study suggests requirement of de novo synthesis of eukaryotic ribosomes for long-term cold acclimation, feedback control of NUC2 and eIF3C2 transcription and links new proteins, AT1G03250, AT5G60530, to plant ribosome biogenesis. We propose that Arabidopsis requires biosynthesis of specialized ribosomes for cold acclimation.Bo Eng CheongOlga Beine-GolovchukMichal GorkaWilliam Wing Ho HoFederico Martinez-SeidelAlexandre Augusto Pereira FirminoAleksandra SkiryczUte RoessnerJoachim KopkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-25 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bo Eng Cheong Olga Beine-Golovchuk Michal Gorka William Wing Ho Ho Federico Martinez-Seidel Alexandre Augusto Pereira Firmino Aleksandra Skirycz Ute Roessner Joachim Kopka Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
description	Abstract Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hyper-accumulation of non-translating subunits at steady-state temperature; after cold-shift, a KCl-sensitive 80S sub-fraction remains depleted. We infer that Arabidopsis may buffer fluctuating translation by pre-existing non-translating ribosomes before de novo synthesis meets temperature-induced demands. Reil1 reil2 double mutants accumulate 43S-preinitiation and pre-60S-maturation complexes and alter paralog composition of ribosomal proteins in non-translating complexes. With few exceptions, e.g. RPL3B and RPL24C, these changes are not under transcriptional control. Our study suggests requirement of de novo synthesis of eukaryotic ribosomes for long-term cold acclimation, feedback control of NUC2 and eIF3C2 transcription and links new proteins, AT1G03250, AT5G60530, to plant ribosome biogenesis. We propose that Arabidopsis requires biosynthesis of specialized ribosomes for cold acclimation.
format	article
author	Bo Eng Cheong Olga Beine-Golovchuk Michal Gorka William Wing Ho Ho Federico Martinez-Seidel Alexandre Augusto Pereira Firmino Aleksandra Skirycz Ute Roessner Joachim Kopka
author_facet	Bo Eng Cheong Olga Beine-Golovchuk Michal Gorka William Wing Ho Ho Federico Martinez-Seidel Alexandre Augusto Pereira Firmino Aleksandra Skirycz Ute Roessner Joachim Kopka
author_sort	Bo Eng Cheong
title	Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_short	Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_full	Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_fullStr	Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_full_unstemmed	Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_sort	arabidopsis rei-like proteins activate ribosome biogenesis during cold acclimation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/710717f23f624354a1201ec1e29d2780
work_keys_str_mv	AT boengcheong arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT olgabeinegolovchuk arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT michalgorka arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT williamwinghoho arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT federicomartinezseidel arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT alexandreaugustopereirafirmino arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT aleksandraskirycz arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT uteroessner arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation AT joachimkopka arabidopsisreilikeproteinsactivateribosomebiogenesisduringcoldacclimation
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Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation

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