Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.

Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postna...

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Autores principales:	Natassya M Noor, Kjeld Møllgård, Benjamin J Wheaton, David L Steer, Jessie S Truettner, Katarzyna M Dziegielewska, W Dalton Dietrich, A Ian Smith, Norman R Saunders
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7108b20080604f5d873452fc9f76638f
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spelling	oai:doaj.org-article:7108b20080604f5d873452fc9f76638f2021-11-18T07:48:20ZExpression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.1932-620310.1371/journal.pone.0062120https://doaj.org/article/7108b20080604f5d873452fc9f76638f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23626776/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postnatal day P35 in control opossums (Monodelphis domestica) and in response to complete spinal transection (T10) at P7, when axonal growth through site of injury occurs, and P28 when this is no longer possible. Cords were collected 1 or 7 days after injury, with age-matched controls and segments rostral to lesion were studied. Following spinal injury ubiquitin levels (western blotting) appeared reduced compared to controls especially one day after injury at P28. In contrast, after injury mRNA expression (qRT-PCR) was slightly increased at P7 but decreased at P28. Changes in isoelectric point of separated ubiquitin indicated possible post-translational modifications. Cellular distribution demonstrated a developmental shift between earliest (P8) and latest (P35) ages examined, from a predominantly cytoplasmic immunoreactivity to a nuclear expression; staining level and shift to nuclear staining was more pronounced following injury, except 7 days after transection at P28. After injury at P7 immunostaining increased in neurons and additionally in oligodendrocytes at P28. Mass spectrometry showed two ubiquitin bands; the heavier was identified as a fusion product, likely to be an ubiquitin precursor. Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets.Natassya M NoorKjeld MøllgårdBenjamin J WheatonDavid L SteerJessie S TruettnerKatarzyna M DziegielewskaW Dalton DietrichA Ian SmithNorman R SaundersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62120 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natassya M Noor Kjeld Møllgård Benjamin J Wheaton David L Steer Jessie S Truettner Katarzyna M Dziegielewska W Dalton Dietrich A Ian Smith Norman R Saunders Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
description	Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postnatal day P35 in control opossums (Monodelphis domestica) and in response to complete spinal transection (T10) at P7, when axonal growth through site of injury occurs, and P28 when this is no longer possible. Cords were collected 1 or 7 days after injury, with age-matched controls and segments rostral to lesion were studied. Following spinal injury ubiquitin levels (western blotting) appeared reduced compared to controls especially one day after injury at P28. In contrast, after injury mRNA expression (qRT-PCR) was slightly increased at P7 but decreased at P28. Changes in isoelectric point of separated ubiquitin indicated possible post-translational modifications. Cellular distribution demonstrated a developmental shift between earliest (P8) and latest (P35) ages examined, from a predominantly cytoplasmic immunoreactivity to a nuclear expression; staining level and shift to nuclear staining was more pronounced following injury, except 7 days after transection at P28. After injury at P7 immunostaining increased in neurons and additionally in oligodendrocytes at P28. Mass spectrometry showed two ubiquitin bands; the heavier was identified as a fusion product, likely to be an ubiquitin precursor. Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets.
format	article
author	Natassya M Noor Kjeld Møllgård Benjamin J Wheaton David L Steer Jessie S Truettner Katarzyna M Dziegielewska W Dalton Dietrich A Ian Smith Norman R Saunders
author_facet	Natassya M Noor Kjeld Møllgård Benjamin J Wheaton David L Steer Jessie S Truettner Katarzyna M Dziegielewska W Dalton Dietrich A Ian Smith Norman R Saunders
author_sort	Natassya M Noor
title	Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
title_short	Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
title_full	Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
title_fullStr	Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
title_full_unstemmed	Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.
title_sort	expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of monodelphis domestica.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7108b20080604f5d873452fc9f76638f
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Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica.

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