Development of an automated system to measure ion channel currents using a surface-modified gold probe

Abstract Artificial lipid bilayer single-channel recording technique has been employed to determine the biophysical and pharmacological properties of various ion channels. However, its measurement efficiency is very low, as it requires two time-consuming processes: preparation of lipid bilayer membr...

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Autores principales:	Minako Hirano, Masahisa Tomita, Chikako Takahashi, Nobuyuki Kawashima, Toru Ide
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7112184ceb324bce85d10222d37eb7e5
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spelling	oai:doaj.org-article:7112184ceb324bce85d10222d37eb7e52021-12-02T19:13:54ZDevelopment of an automated system to measure ion channel currents using a surface-modified gold probe10.1038/s41598-021-97237-z2045-2322https://doaj.org/article/7112184ceb324bce85d10222d37eb7e52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97237-zhttps://doaj.org/toc/2045-2322Abstract Artificial lipid bilayer single-channel recording technique has been employed to determine the biophysical and pharmacological properties of various ion channels. However, its measurement efficiency is very low, as it requires two time-consuming processes: preparation of lipid bilayer membranes and incorporation of ion channels into the membranes. In order to address these problems, we previously developed a technique based on hydrophilically modified gold probes on which are immobilized ion channels that can be promptly incorporated into the bilayer membrane at the same time as the membrane is formed on the probes’ hydrophilic area. Here, we improved further this technique by optimizing the gold probe and developed an automated channel current measurement system. We found that use of probes with rounded tips enhanced the efficiency of channel current measurements, and introducing a hydrophobic area on the probe surface, beside the hydrophilic one, further increased measurement efficiency by boosting membrane stability. Moreover, we developed an automated measurement system using the optimized probes; it enabled us to automatically measure channel currents and analyze the effects of a blocker on channel activity. Our study will contribute to the development of high-throughput devices to identify drug candidates affecting ion channel activity.Minako HiranoMasahisa TomitaChikako TakahashiNobuyuki KawashimaToru IdeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Minako Hirano Masahisa Tomita Chikako Takahashi Nobuyuki Kawashima Toru Ide Development of an automated system to measure ion channel currents using a surface-modified gold probe
description	Abstract Artificial lipid bilayer single-channel recording technique has been employed to determine the biophysical and pharmacological properties of various ion channels. However, its measurement efficiency is very low, as it requires two time-consuming processes: preparation of lipid bilayer membranes and incorporation of ion channels into the membranes. In order to address these problems, we previously developed a technique based on hydrophilically modified gold probes on which are immobilized ion channels that can be promptly incorporated into the bilayer membrane at the same time as the membrane is formed on the probes’ hydrophilic area. Here, we improved further this technique by optimizing the gold probe and developed an automated channel current measurement system. We found that use of probes with rounded tips enhanced the efficiency of channel current measurements, and introducing a hydrophobic area on the probe surface, beside the hydrophilic one, further increased measurement efficiency by boosting membrane stability. Moreover, we developed an automated measurement system using the optimized probes; it enabled us to automatically measure channel currents and analyze the effects of a blocker on channel activity. Our study will contribute to the development of high-throughput devices to identify drug candidates affecting ion channel activity.
format	article
author	Minako Hirano Masahisa Tomita Chikako Takahashi Nobuyuki Kawashima Toru Ide
author_facet	Minako Hirano Masahisa Tomita Chikako Takahashi Nobuyuki Kawashima Toru Ide
author_sort	Minako Hirano
title	Development of an automated system to measure ion channel currents using a surface-modified gold probe
title_short	Development of an automated system to measure ion channel currents using a surface-modified gold probe
title_full	Development of an automated system to measure ion channel currents using a surface-modified gold probe
title_fullStr	Development of an automated system to measure ion channel currents using a surface-modified gold probe
title_full_unstemmed	Development of an automated system to measure ion channel currents using a surface-modified gold probe
title_sort	development of an automated system to measure ion channel currents using a surface-modified gold probe
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7112184ceb324bce85d10222d37eb7e5
work_keys_str_mv	AT minakohirano developmentofanautomatedsystemtomeasureionchannelcurrentsusingasurfacemodifiedgoldprobe AT masahisatomita developmentofanautomatedsystemtomeasureionchannelcurrentsusingasurfacemodifiedgoldprobe AT chikakotakahashi developmentofanautomatedsystemtomeasureionchannelcurrentsusingasurfacemodifiedgoldprobe AT nobuyukikawashima developmentofanautomatedsystemtomeasureionchannelcurrentsusingasurfacemodifiedgoldprobe AT toruide developmentofanautomatedsystemtomeasureionchannelcurrentsusingasurfacemodifiedgoldprobe
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Development of an automated system to measure ion channel currents using a surface-modified gold probe

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