Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells

Abstract Periodontal diseases manifest by the formation of deep pockets between the gingiva and teeth where multispecies bacterial biofilms flourish, causing inflammation and bone loss. Epithelial cell receptor αvβ6 integrin that regulates inflammation by activating the anti-inflammatory cytokine tr...

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Autores principales: Jiarui Bi, Leeni Koivisto, Aihui Pang, Ming Li, Guoqiao Jiang, Saljae Aurora, Zhejun Wang, Gethin R. Owen, Jiayin Dai, Ya Shen, Daniel Grenier, Markus Haapasalo, Lari Häkkinen, Hannu Larjava
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/7122099892e944fca08d2c0010fd7dae
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spelling oai:doaj.org-article:7122099892e944fca08d2c0010fd7dae2021-12-02T12:31:52ZSuppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells10.1038/s41598-017-03619-72045-2322https://doaj.org/article/7122099892e944fca08d2c0010fd7dae2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03619-7https://doaj.org/toc/2045-2322Abstract Periodontal diseases manifest by the formation of deep pockets between the gingiva and teeth where multispecies bacterial biofilms flourish, causing inflammation and bone loss. Epithelial cell receptor αvβ6 integrin that regulates inflammation by activating the anti-inflammatory cytokine transforming growth factor-β1, is highly expressed in healthy junctional epithelium that connects the gingiva to the tooth enamel. However, its expression is attenuated in human periodontal disease. Moreover, Itgb6 −/− mice display increased periodontal inflammation compared to wild-type mice. We hypothesized that bacterial biofilms present in the periodontal pockets suppress αvβ6 integrin levels in periodontal disease and that this change aggravates inflammation. To this end, we generated three-week-old multi-species oral biofilms in vitro and treated cultured gingival epithelial cells (GECs) with their extracts. The biofilm extracts caused suppression of β6 integrin expression and upregulation of pro-inflammatory cytokines, including interleukin-1β and -6. Furthermore, GECs with β6 integrin siRNA knockdown showed increased interleukin-1β expression, indicating that αvβ6 integrin-deficiency is associated with pro-inflammatory cytokine responsiveness. FSL-1, a synthetic bacterial lipopeptide, also suppressed β6 integrin expression in GECs. Therefore, biofilm components, including lipopeptides, may downregulate αvβ6 integrin expression in the pocket epithelium and thus promote epithelial cell-driven pro-inflammatory response in periodontal disease.Jiarui BiLeeni KoivistoAihui PangMing LiGuoqiao JiangSaljae AuroraZhejun WangGethin R. OwenJiayin DaiYa ShenDaniel GrenierMarkus HaapasaloLari HäkkinenHannu LarjavaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jiarui Bi
Leeni Koivisto
Aihui Pang
Ming Li
Guoqiao Jiang
Saljae Aurora
Zhejun Wang
Gethin R. Owen
Jiayin Dai
Ya Shen
Daniel Grenier
Markus Haapasalo
Lari Häkkinen
Hannu Larjava
Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
description Abstract Periodontal diseases manifest by the formation of deep pockets between the gingiva and teeth where multispecies bacterial biofilms flourish, causing inflammation and bone loss. Epithelial cell receptor αvβ6 integrin that regulates inflammation by activating the anti-inflammatory cytokine transforming growth factor-β1, is highly expressed in healthy junctional epithelium that connects the gingiva to the tooth enamel. However, its expression is attenuated in human periodontal disease. Moreover, Itgb6 −/− mice display increased periodontal inflammation compared to wild-type mice. We hypothesized that bacterial biofilms present in the periodontal pockets suppress αvβ6 integrin levels in periodontal disease and that this change aggravates inflammation. To this end, we generated three-week-old multi-species oral biofilms in vitro and treated cultured gingival epithelial cells (GECs) with their extracts. The biofilm extracts caused suppression of β6 integrin expression and upregulation of pro-inflammatory cytokines, including interleukin-1β and -6. Furthermore, GECs with β6 integrin siRNA knockdown showed increased interleukin-1β expression, indicating that αvβ6 integrin-deficiency is associated with pro-inflammatory cytokine responsiveness. FSL-1, a synthetic bacterial lipopeptide, also suppressed β6 integrin expression in GECs. Therefore, biofilm components, including lipopeptides, may downregulate αvβ6 integrin expression in the pocket epithelium and thus promote epithelial cell-driven pro-inflammatory response in periodontal disease.
format article
author Jiarui Bi
Leeni Koivisto
Aihui Pang
Ming Li
Guoqiao Jiang
Saljae Aurora
Zhejun Wang
Gethin R. Owen
Jiayin Dai
Ya Shen
Daniel Grenier
Markus Haapasalo
Lari Häkkinen
Hannu Larjava
author_facet Jiarui Bi
Leeni Koivisto
Aihui Pang
Ming Li
Guoqiao Jiang
Saljae Aurora
Zhejun Wang
Gethin R. Owen
Jiayin Dai
Ya Shen
Daniel Grenier
Markus Haapasalo
Lari Häkkinen
Hannu Larjava
author_sort Jiarui Bi
title Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
title_short Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
title_full Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
title_fullStr Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
title_full_unstemmed Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells
title_sort suppression of αvβ6 integrin expression by polymicrobial oral biofilms in gingival epithelial cells
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/7122099892e944fca08d2c0010fd7dae
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