Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells

Abstract Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play a key role in purinergic signaling in the nervous system in both normal and pathological conditions. In the retina, particularly high levels of Panx1 are found in retinal ganglion cells (RGCs), but the normal physiological f...

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Autores principales:	Galina Dvoriantchikova, Alexey Pronin, Sarah Kurtenbach, Abduqodir Toychiev, Tsung-Han Chou, Christopher W. Yee, Breanne Prindeville, Junior Tayou, Vittorio Porciatti, Botir T. Sagdullaev, Vladlen Z. Slepak, Valery I. Shestopalov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71240551117045f9a0c3465da7d4dcc1
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spelling	oai:doaj.org-article:71240551117045f9a0c3465da7d4dcc12021-12-02T15:08:34ZPannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells10.1038/s41598-018-23894-22045-2322https://doaj.org/article/71240551117045f9a0c3465da7d4dcc12018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23894-2https://doaj.org/toc/2045-2322Abstract Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play a key role in purinergic signaling in the nervous system in both normal and pathological conditions. In the retina, particularly high levels of Panx1 are found in retinal ganglion cells (RGCs), but the normal physiological function in these cells remains unclear. In this study, we used patch clamp recordings in the intact inner retina to show that evoked currents characteristic of Panx1 channel activity were detected only in RGCs, particularly in the OFF-type cells. The analysis of pattern electroretinogram (PERG) recordings indicated that Panx1 contributes to the electrical output of the retina. Consistently, PERG amplitudes were significantly impaired in the eyes with targeted ablation of the Panx1 gene in RGCs. Under ocular hypertension and ischemic conditions, however, high Panx1 activity permeated cell membranes and facilitated the selective loss of RGCs or stably transfected Neuro2A cells. Our results show that high expression of the Panx1 channel in RGCs is essential for visual function in the inner retina but makes these cells highly sensitive to mechanical and ischemic stresses. These findings are relevant to the pathophysiology of retinal disorders induced by increased intraocular pressure, such as glaucoma.Galina DvoriantchikovaAlexey ProninSarah KurtenbachAbduqodir ToychievTsung-Han ChouChristopher W. YeeBreanne PrindevilleJunior TayouVittorio PorciattiBotir T. SagdullaevVladlen Z. SlepakValery I. ShestopalovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Galina Dvoriantchikova Alexey Pronin Sarah Kurtenbach Abduqodir Toychiev Tsung-Han Chou Christopher W. Yee Breanne Prindeville Junior Tayou Vittorio Porciatti Botir T. Sagdullaev Vladlen Z. Slepak Valery I. Shestopalov Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
description	Abstract Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play a key role in purinergic signaling in the nervous system in both normal and pathological conditions. In the retina, particularly high levels of Panx1 are found in retinal ganglion cells (RGCs), but the normal physiological function in these cells remains unclear. In this study, we used patch clamp recordings in the intact inner retina to show that evoked currents characteristic of Panx1 channel activity were detected only in RGCs, particularly in the OFF-type cells. The analysis of pattern electroretinogram (PERG) recordings indicated that Panx1 contributes to the electrical output of the retina. Consistently, PERG amplitudes were significantly impaired in the eyes with targeted ablation of the Panx1 gene in RGCs. Under ocular hypertension and ischemic conditions, however, high Panx1 activity permeated cell membranes and facilitated the selective loss of RGCs or stably transfected Neuro2A cells. Our results show that high expression of the Panx1 channel in RGCs is essential for visual function in the inner retina but makes these cells highly sensitive to mechanical and ischemic stresses. These findings are relevant to the pathophysiology of retinal disorders induced by increased intraocular pressure, such as glaucoma.
format	article
author	Galina Dvoriantchikova Alexey Pronin Sarah Kurtenbach Abduqodir Toychiev Tsung-Han Chou Christopher W. Yee Breanne Prindeville Junior Tayou Vittorio Porciatti Botir T. Sagdullaev Vladlen Z. Slepak Valery I. Shestopalov
author_facet	Galina Dvoriantchikova Alexey Pronin Sarah Kurtenbach Abduqodir Toychiev Tsung-Han Chou Christopher W. Yee Breanne Prindeville Junior Tayou Vittorio Porciatti Botir T. Sagdullaev Vladlen Z. Slepak Valery I. Shestopalov
author_sort	Galina Dvoriantchikova
title	Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
title_short	Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
title_full	Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
title_fullStr	Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
title_full_unstemmed	Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
title_sort	pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/71240551117045f9a0c3465da7d4dcc1
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Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells

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