Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Lycoris sprengeri Bulblet Regeneration In Vitro

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For Lycoris species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of Lycoris sprengeri...

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Autores principales:	Ziming Ren, Yunchen Xu, Xuesi Lvy, Dong Zhang, Cong Gao, Yefan Lin, Yue Liu, Yun Wu, Yiping Xia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	6-benzyladenine bulblet regeneration sucrose cleavage endogenous hormone cell wall invertase <i>Lycoris</i> <i>sprengeri</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7124da62c8474752b414798ccb9affe3
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spelling	oai:doaj.org-article:7124da62c8474752b414798ccb9affe32021-11-11T17:18:26ZEarly Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro10.3390/ijms2221118901422-00671661-6596https://doaj.org/article/7124da62c8474752b414798ccb9affe32021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11890https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i> (<i>Ls</i>) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of <i>LsCWIN2</i> than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.Ziming RenYunchen XuXuesi LvyDong ZhangCong GaoYefan LinYue LiuYun WuYiping XiaMDPI AGarticle6-benzyladeninebulblet regenerationsucrose cleavageendogenous hormonecell wall invertase<i>Lycoris</i> <i>sprengeri</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11890, p 11890 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	6-benzyladenine bulblet regeneration sucrose cleavage endogenous hormone cell wall invertase <i>Lycoris</i> <i>sprengeri</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	6-benzyladenine bulblet regeneration sucrose cleavage endogenous hormone cell wall invertase <i>Lycoris</i> <i>sprengeri</i> Biology (General) QH301-705.5 Chemistry QD1-999 Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
description	Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i> (<i>Ls</i>) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of <i>LsCWIN2</i> than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.
format	article
author	Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia
author_facet	Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia
author_sort	Ziming Ren
title	Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
title_short	Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
title_full	Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
title_fullStr	Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
title_full_unstemmed	Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
title_sort	early sucrose degradation and the dominant sucrose cleavage pattern influence <i>lycoris sprengeri</i> bulblet regeneration in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7124da62c8474752b414798ccb9affe3
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Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro

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