Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo

Abstract Nonhuman primates provide an important model not only for understanding human brain but also for translational research in neurological and psychiatric disorders. However, many high-resolution techniques for recording neural activity in vivo that were initially established for rodents have...

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Autores principales:	Ran Ding, Xiang Liao, Jingcheng Li, Jianxiong Zhang, Meng Wang, Yu Guang, Han Qin, Xingyi Li, Kuan Zhang, Shanshan Liang, Jiangheng Guan, Jia Lou, Hongbo Jia, Bingbo Chen, Hui Shen, Xiaowei Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7127d384d9a24af4aed5505e83d80791
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spelling	oai:doaj.org-article:7127d384d9a24af4aed5505e83d807912021-12-02T11:52:56ZTargeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo10.1038/s41598-017-03105-02045-2322https://doaj.org/article/7127d384d9a24af4aed5505e83d807912017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03105-0https://doaj.org/toc/2045-2322Abstract Nonhuman primates provide an important model not only for understanding human brain but also for translational research in neurological and psychiatric disorders. However, many high-resolution techniques for recording neural activity in vivo that were initially established for rodents have not been yet applied to the nonhuman primate brain. Here, we introduce a combination of two-photon targeted patching and dendritic Ca2+ imaging to the neocortex of adult common marmoset, an invaluable primate model for neuroscience research. Using targeted patching, we show both spontaneous and sensory-evoked intracellular dynamics of visually identified neurons in the marmoset cortex. Using two-photon Ca2+ imaging and intracellular pharmacological manipulation, we report both action-potential-associated global and synaptically-evoked NMDA (N-methyl-D-aspartate) receptor-mediated local Ca2+ signals in dendrites and spines of the superficial-layer cortical neurons. Therefore, we demonstrate the presence of synaptic Ca2+ signals in neuronal dendrites in living nonhuman primates. This work represents a proof-of-principle for exploring the primate brain functions in vivo by monitoring neural activity and morphology at a subcellular resolution.Ran DingXiang LiaoJingcheng LiJianxiong ZhangMeng WangYu GuangHan QinXingyi LiKuan ZhangShanshan LiangJiangheng GuanJia LouHongbo JiaBingbo ChenHui ShenXiaowei ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ran Ding Xiang Liao Jingcheng Li Jianxiong Zhang Meng Wang Yu Guang Han Qin Xingyi Li Kuan Zhang Shanshan Liang Jiangheng Guan Jia Lou Hongbo Jia Bingbo Chen Hui Shen Xiaowei Chen Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
description	Abstract Nonhuman primates provide an important model not only for understanding human brain but also for translational research in neurological and psychiatric disorders. However, many high-resolution techniques for recording neural activity in vivo that were initially established for rodents have not been yet applied to the nonhuman primate brain. Here, we introduce a combination of two-photon targeted patching and dendritic Ca2+ imaging to the neocortex of adult common marmoset, an invaluable primate model for neuroscience research. Using targeted patching, we show both spontaneous and sensory-evoked intracellular dynamics of visually identified neurons in the marmoset cortex. Using two-photon Ca2+ imaging and intracellular pharmacological manipulation, we report both action-potential-associated global and synaptically-evoked NMDA (N-methyl-D-aspartate) receptor-mediated local Ca2+ signals in dendrites and spines of the superficial-layer cortical neurons. Therefore, we demonstrate the presence of synaptic Ca2+ signals in neuronal dendrites in living nonhuman primates. This work represents a proof-of-principle for exploring the primate brain functions in vivo by monitoring neural activity and morphology at a subcellular resolution.
format	article
author	Ran Ding Xiang Liao Jingcheng Li Jianxiong Zhang Meng Wang Yu Guang Han Qin Xingyi Li Kuan Zhang Shanshan Liang Jiangheng Guan Jia Lou Hongbo Jia Bingbo Chen Hui Shen Xiaowei Chen
author_facet	Ran Ding Xiang Liao Jingcheng Li Jianxiong Zhang Meng Wang Yu Guang Han Qin Xingyi Li Kuan Zhang Shanshan Liang Jiangheng Guan Jia Lou Hongbo Jia Bingbo Chen Hui Shen Xiaowei Chen
author_sort	Ran Ding
title	Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
title_short	Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
title_full	Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
title_fullStr	Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
title_full_unstemmed	Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo
title_sort	targeted patching and dendritic ca2+ imaging in nonhuman primate brain in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7127d384d9a24af4aed5505e83d80791
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_version_	1718394931248103424

Targeted Patching and Dendritic Ca2+ Imaging in Nonhuman Primate Brain in vivo

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