Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi...

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Autores principales:	Popović Tatjana, Mitrović Petar, Kosovac Andrea
Formato:	article
Lenguaje:	EN SR
Publicado:	Institute of field and vegetable crops, Novi Sad 2021
Materias:	celery molecular characterization stolbur phytoplasma Plant culture SB1-1110 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/71292f023d294672b4dfa4440a2f97f9
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spelling	oai:doaj.org-article:71292f023d294672b4dfa4440a2f97f92021-12-05T21:31:45ZMolecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog1821-39442217-839210.5937/ratpov58-33227https://doaj.org/article/71292f023d294672b4dfa4440a2f97f92021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2021/1821-39442102066P.pdfhttps://doaj.org/toc/1821-3944https://doaj.org/toc/2217-8392'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.Popović TatjanaMitrović PetarKosovac AndreaInstitute of field and vegetable crops, Novi Sadarticlecelerymolecular characterizationstolbur phytoplasmaPlant cultureSB1-1110BiotechnologyTP248.13-248.65ENSRRatarstvo i Povrtarstvo, Vol 58, Iss 2, Pp 66-71 (2021)
institution	DOAJ
collection	DOAJ
language	EN SR
topic	celery molecular characterization stolbur phytoplasma Plant culture SB1-1110 Biotechnology TP248.13-248.65
spellingShingle	celery molecular characterization stolbur phytoplasma Plant culture SB1-1110 Biotechnology TP248.13-248.65 Popović Tatjana Mitrović Petar Kosovac Andrea Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
description	'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.
format	article
author	Popović Tatjana Mitrović Petar Kosovac Andrea
author_facet	Popović Tatjana Mitrović Petar Kosovac Andrea
author_sort	Popović Tatjana
title	Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
title_short	Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
title_full	Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
title_fullStr	Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
title_full_unstemmed	Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
title_sort	molecular characterization of 'candidatus phytoplasma solani' in celery: case study in futog
publisher	Institute of field and vegetable crops, Novi Sad
publishDate	2021
url	https://doaj.org/article/71292f023d294672b4dfa4440a2f97f9
work_keys_str_mv	AT popovictatjana molecularcharacterizationofcandidatusphytoplasmasolaniincelerycasestudyinfutog AT mitrovicpetar molecularcharacterizationofcandidatusphytoplasmasolaniincelerycasestudyinfutog AT kosovacandrea molecularcharacterizationofcandidatusphytoplasmasolaniincelerycasestudyinfutog
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Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

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